Nagafuchi S, Seki S, Nakahori Y, Tamura T, Numabe H, Nakagome Y
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.
Jpn J Hum Genet. 1992 Sep;37(3):187-93. doi: 10.1007/BF01900712.
Three probes each detecting a locus on the proximal long arm of the Y chromosome were partially sequenced. Thus, 3 sets of novel primers were developed which enable PCR detection of these 3 loci. Five previously reported primer sets, 3 on the short arm and each one on the centromere and the distal long arm, were mapped along with the novel three using a mapping panel consisted of 8 patients each with different structural abnormality of the Y chromosome. Now, PCR detection of these 8 loci covering an entire length of the Y chromosome has become possible enabling rapid screening of patients with Y chromosome aberrations.
对三个分别检测Y染色体近端长臂上一个位点的探针进行了部分测序。因此，开发了3组新型引物，可用于对这3个位点进行PCR检测。使用由8名Y染色体结构异常各异的患者组成的定位板，将5组先前报道的引物（3组位于短臂，每组分别位于着丝粒和远端长臂）与新的3组引物一起进行了定位。现在，已能够对覆盖Y染色体全长的这8个位点进行PCR检测，从而能够快速筛查Y染色体畸变患者。
