Villegas Juan
Department of Anatomy and Cell Biology, School of Medicine, University of Cantabria, C/Cardenal Herrera Oria s/n, 39011 Santander, Cantabria, Spain.
Cell Tissue Res. 2004 Mar;315(3):349-59. doi: 10.1007/s00441-003-0845-7. Epub 2004 Jan 15.
Angiogenesis and reperfusion of blood vessels were analysed qualitatively, at the light- and electron-microscopical levels, in solid pineal autografts placed intracerebrally in adult rats (post-transplantation survival times: 1, 3, 7, 10, 14 and 28 days). Reperfusion of blood vessels was studied in sections from immersion-fixed brains incubated to demonstrate the endogenous peroxidase activity of erythrocytes within the lumen of blood vessels. The possible presence of the blood-brain barrier (BBB) within the grafts was also investigated by injecting native horseradish peroxidase (HRP) intravenously into the rats. Angiogenesis, the morphological and functional properties of blood vessels vascularizing the grafts and the survival of pineal tissue were analysed ultrastructurally following transplantation. Revascularization of pineal autografts placed into the adult host central nervous system occurred very slowly, requiring 7-10 days to establish anastomoses between graft and host blood vessels. During this process, signs of angiogenesis in pineal and cerebral capillaries were evident, suggesting that both contributed to graft revascularization. Morphological and functional studies with HRP revealed that, following transplantation, blood vessels at the graft-host interface or within pineal autografts maintained their morphological and functional properties: they were fenestrated and did not present a BBB to blood-borne peroxidase. Thus, after grafting, the presence or absence of the BBB is graft-determined. Revascularized pineal tissue showed good survival and pinealocytes revealed structural features of active secretory cells.
在成年大鼠脑内植入的实体松果体自体移植物中（移植后存活时间为1、3、7、10、14和28天），在光镜和电镜水平对血管生成和血管再灌注进行了定性分析。通过对浸没固定的脑切片进行孵育以显示血管腔内红细胞的内源性过氧化物酶活性，研究血管再灌注情况。还通过向大鼠静脉注射天然辣根过氧化物酶（HRP）来研究移植物中血脑屏障（BBB）的可能存在情况。移植后，通过超微结构分析血管生成、为移植物供血的血管的形态和功能特性以及松果体组织的存活情况。植入成年宿主中枢神经系统的松果体自体移植物的血管再通非常缓慢，需要7 - 10天才能在移植物和宿主血管之间建立吻合。在此过程中，松果体和脑毛细血管中的血管生成迹象明显，表明两者都有助于移植物的血管再通。用HRP进行的形态学和功能研究表明，移植后，移植物 - 宿主界面或松果体自体移植物内的血管保持其形态和功能特性：它们有窗孔，对血源性过氧化物酶不呈现血脑屏障。因此，移植后，血脑屏障的存在与否由移植物决定。血管再通的松果体组织显示出良好的存活情况，松果体细胞呈现出活跃分泌细胞的结构特征。
