利用贾菲反应进行人血清中肌酐测定的多元校准。

Multiway calibration for creatinine determination in human serum using the Jaffé reaction.

作者信息

Guterres Marcos V, Volpe Pedro O L, Ferreira Márcia M C

机构信息

Department of Physical Chemistry, Institute of Chemistry, Universidade Estadual de Crampinas, SP, 13083-970, Brazil.

出版信息

Appl Spectrosc. 2004 Jan;58(1):54-60. doi: 10.1366/000370204322729478.

DOI:10.1366/000370204322729478

PMID:14727721

Abstract

Second-order calibration and multivariate spectroscopic-kinetic measurements in the visible region are proposed to improve the Jaffé method for creatinine assay. Analyses performed on synthetic mixtures containing bilirubin, glucose, and albumin confirm that second-order calibration is useful for creatinine determination in human serum. Quantitative determinations of creatinine with the parallel factor analysis (PARAFAC) and direct trilinear decomposition (TLD) methods were compared. It is shown that both methods can be used for creatinine determination in human serum, with an SEP (standard error of prediction) of 2.22 and coefficient of variability of 6.14% for PARAFAC, and an SEP of 2.38 and coefficient of variability of 6.57% for TLD [corrected].

摘要

本文提出在可见光区域进行二阶校正和多变量光谱动力学测量，以改进用于肌酐测定的杰氏法。对含有胆红素、葡萄糖和白蛋白的合成混合物进行的分析证实，二阶校正对于人血清中肌酐的测定是有用的。比较了用平行因子分析（PARAFAC）和直接三线分解（TLD）方法对肌酐进行的定量测定。结果表明，两种方法均可用于人血清中肌酐的测定，PARAFAC法的预测标准误差（SEP）为2.22，变异系数为6.14%，TLD法的SEP为2.38，变异系数为6.57%[校正后]。