Calafell J M, Nogués C, Ponsà M, Santaló J, Egozcue J
Dept. Biologia Cel.lular i Fisiologia, Universitat Autònoma de Barcelona, Spain.
J Assist Reprod Genet. 1992 Aug;9(4):365-72. doi: 10.1007/BF01203961.
The aim of this work was to determine the morphology of the zona pellucida surface of immature and in vitro matured mouse oocytes by scanning electron microscopy. For this purpose two groups of immature oocytes (germinal vesicle group and metaphase I group) were studied either before or after in vitro maturation.
Before in vitro maturation, the germinal vesicle immature group showed mainly an unstructured zona pellucida surface with smooth cumulus cells. The metaphase I immature group showed a more structured zona pellucida with smooth or blebbing cumulus cells. After in vitro maturation, development of the zona pellucida toward a mature surface, related to the initial degree of oocyte maturity, was observed in both groups.
These observations show a correlation between the morphology of the zona pellucida surface and the degree of oocyte maturity; the in vitro maturation process can give rise to a proper development of this endowment when immature oocytes are used.
本研究旨在通过扫描电子显微镜确定未成熟和体外成熟小鼠卵母细胞透明带表面的形态。为此，研究了两组未成熟卵母细胞（生发泡期组和减数分裂中期I组）在体外成熟前后的情况。
在体外成熟前，生发泡期未成熟组的透明带表面主要无结构，卵丘细胞光滑。减数分裂中期I未成熟组的透明带结构更明显，卵丘细胞光滑或有泡状突起。体外成熟后，两组均观察到透明带向成熟表面发展，这与卵母细胞的初始成熟程度有关。
这些观察结果表明透明带表面形态与卵母细胞成熟程度之间存在相关性；当使用未成熟卵母细胞时，体外成熟过程可使这种结构正常发育。
