Wu Yu-lian, Yu Jun-xiu, Shen Hong-wei, Han Bing, Gao Shun-liang
Department of Surgery, Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310009, China.
Zhonghua Wai Ke Za Zhi. 2003 Dec;41(12):928-31.
To evaluate the value of serum TR(6) for the diagnosis and TNM classification in patients with gastric carcinoma.
Serum TR(6) levels were measured using ELISA method in 31 gastric cancer patients, 19 patients with nonmalignant conditions and 29 healthy individuals. TR(6) expression in tumor mass was studied with immunohistochemistry. TR(6) gene copy number in tumor tissues was evaluated by real time PCR.
Ninety-seven point nine percent (47 of 48 cases) of healthy individuals and patients with nonmalignant conditions were serum TR(6)-negative. In contrast, 71% (22 of 31 cases) of gastric cancer patients were serum TR(6)-positive. Serum TR(6) positiveness was closely correlated with tumor differentiation status and TNM classification. TR(6) gene amplification did not occur in gastric carcinoma.
Serum TR(6) levels were correlated significantly with TNM stage and histopathological type of tumor. This can help to determine the pre-operative TNM classification and to choose the optimal extent of lymph node dissection for gastric cancer.
评估血清TR(6)在胃癌患者诊断及TNM分期中的价值。
采用ELISA法检测31例胃癌患者、19例非恶性疾病患者及29例健康个体的血清TR(6)水平。采用免疫组织化学法研究肿瘤组织中TR(6)的表达。通过实时PCR评估肿瘤组织中TR(6)基因拷贝数。
97.9%（48例中的47例）健康个体及非恶性疾病患者血清TR(6)呈阴性。相比之下，71%（31例中的22例）胃癌患者血清TR(6)呈阳性。血清TR(6)阳性与肿瘤分化状态及TNM分期密切相关。胃癌中未发生TR(6)基因扩增。
血清TR(6)水平与肿瘤的TNM分期及组织病理学类型显著相关。这有助于确定术前TNM分期，并为胃癌选择最佳的淋巴结清扫范围。
