[激活素A、卵泡抑素与子痫前期关系的研究]

[Study on the relationship between activin A, follistatin and preeclampsia].

作者信息

Zhao Liang, Shang Tao, Wang Yan-ling, Tang Shuang, Li Hui, Liu Zhong-hui

机构信息

Department of Obstetrics and Gynecology, Second College of China Medical University, Shenyang 110004, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2003 Nov;38(11):676-9.

PMID:14728855

Abstract

OBJECTIVE

The purpose of this study was to determine the relationship of maternal serum activin A, follistatin and their mRNA expression in placenta with preeclampsia at term.

METHODS

Twenty women with preeclampsia were enrolled in this study. Twenty healthy pregnancies matched for gestational ages were recruited as control group. Maternal serum activin A and follistatin in two groups were compared by using enzyme linked immunosorbent assay (ELISA). Placental tissues were obtained from preeclampsia and healthy control groups for analysis of activin A mRNA and follistatin mRNA expression by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Linear correlation analysis was used to explore the relationship between placental avtivin A mRNA and maternal serum activin A concentrations.

RESULTS

(1) Maternal serum concentrations of activin A are significantly increased in preeclampsia group (33.7 +/- 6.6) micro g/L compared with control group (9.9 +/- 2.1) micro g/L (P < 0.01). Maternal serum follistatin levels were not different between preeclampsia group (5.1 +/- 0.6) micro g/L and control group (4.7 +/- 0.3) micro g/L (P > 0.05). (2) The placentas of preeclampsia group expressed activin A mRNA levels at significantly higher levels 1.11 +/- 0.21 than that of control group 0.61 +/- 0.17 (P < 0.01). The expression of follistatin mRNA were not different between preeclampsia group 0.57 +/- 0.31 and control group 0.54 +/- 0.27 (P > 0.05). (3) In preeclampsia and control groups, maternal serum activin A concentrations had a significant positive correlation with activin A mRNA expression in placentas (r = 0.89, P < 0.01).

CONCLUSION

These results suggest that activin A is increased in women with preeclampsia, the elevated levels of activin A due to increased placental production.

摘要

目的

本研究旨在确定足月子痫前期孕妇血清激活素A、卵泡抑素及其在胎盘中的mRNA表达之间的关系。

方法

本研究纳入20例子痫前期孕妇。选取20例孕周匹配的健康孕妇作为对照组。采用酶联免疫吸附测定（ELISA）法比较两组孕妇血清激活素A和卵泡抑素水平。采集子痫前期组和健康对照组的胎盘组织，采用定量逆转录-聚合酶链反应（RT-PCR）分析激活素A mRNA和卵泡抑素mRNA的表达。采用线性相关分析探讨胎盘激活素A mRNA与孕妇血清激活素A浓度之间的关系。

结果

（1）子痫前期组孕妇血清激活素A浓度显著高于对照组，子痫前期组为（33.7±6.6）μg/L，对照组为（9.9±2.1）μg/L（P<0.01）。子痫前期组孕妇血清卵泡抑素水平与对照组比较，差异无统计学意义，子痫前期组为（5.1±0.6）μg/L，对照组为（4.7±0.3）μg/L（P>0.05）。（2）子痫前期组胎盘激活素A mRNA表达水平显著高于对照组，子痫前期组为1.11±0.21，对照组为0.61±0.17（P<0.01）。子痫前期组卵泡抑素mRNA表达与对照组比较，差异无统计学意义，子痫前期组为0.57±0.31，对照组为0.54±0.27（P>0.05）。（3）子痫前期组和对照组中，孕妇血清激活素A浓度与胎盘激活素A mRNA表达呈显著正相关（r=0.89，P<0.01）。