Han Li-ping, Qiao Yu-huan, Dong Zi-ming, Shi Hui-rong, Zhao Guo-qiang, Liu Dong
Department of Obstetrics and Gynecology, the First Affliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Fu Chan Ke Za Zhi. 2003 Oct;38(10):618-20.
To investigate whether DNA polymerase beta (POLB) gene mutations occur in human cervical cancer (CC).
To collect fresh specimens from 34 cervical cancer and examine the mutation of POLB gene using reverse transcription polymerase chain reaction (RT-PCR), single strand conformation polymorphism (SSCP) analysis and sequence analysis. According to their histological grading, the 34 cases of CC were divided into three groups: 9 cases of grade I (G(1)), 14 cases of grade II (G(2)), 11 cases of grade III (G(3)).
POLB mutations were detected in the tissues of CC. The mutation of POLB is related with the histological differentiated of the CC. The mutated rates in low differentiated cancer were significantly higher than those in moderate and high ones, and the difference was significant (P < 0.05). The sequencing of the PCR product showed an A to G chang at nucleotide 660, which resulted in a substitution of the amino acid from arginine to glycin at codon 182.
There were POLB mutations in the tissues of CC, and this may be related with the development of the CC. The mutations impaired the catalytic activity of POLB. As a result, this may lead to observed accumulation of mutations in tumor cells.
研究人类宫颈癌（CC）中是否发生DNA聚合酶β（POLB）基因突变。
收集34例宫颈癌新鲜标本，采用逆转录聚合酶链反应（RT-PCR）、单链构象多态性（SSCP）分析及序列分析检测POLB基因的突变情况。根据组织学分级，将34例CC患者分为三组：I级（G(1)）9例，II级（G(2)）14例，III级（G(3)）11例。
在CC组织中检测到POLB突变。POLB突变与CC的组织学分化有关。低分化癌的突变率显著高于中、高分化癌，差异有统计学意义（P < 0.05）。PCR产物测序显示第660位核苷酸由A变为G，导致第182密码子的氨基酸由精氨酸替换为甘氨酸。
CC组织中存在POLB突变，这可能与CC的发生发展有关。这些突变损害了POLB的催化活性。因此，这可能导致肿瘤细胞中观察到的突变积累。
