Maurer Martin H, Canis Martin, Kuschinsky Wolfgang, Duelli Roman
Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany.
Neurosci Lett. 2004 Jan 23;355(1-2):105-8. doi: 10.1016/j.neulet.2003.10.056.
Monocarboxylate transporters type 1 (MCT1) facilitate the transport of monocarboxylates across cell membranes of the blood-brain barrier and brain parenchymal cells. The present study had two aims: (1) to determine the local distribution of MCT1 in the brain; and (2) to compare the local densities of MCT1 with the local densities of the main nutritional transporters, glucose transporter GLUT1. Using immunoautoradiography of cryosections from rat brain, 32 brain structures were analyzed. (1) A heterogenous distribution pattern of MCT1 densities was observed throughout the brain. Compared to brain homogenate (100%), MCT1 densities ranged from 43 to 164% in the brain structures investigated. Local GLUT1 densities showed a comparable range (35-145%). (2) A close correlation was found between local MCT1 and local GLUT1 densities. As local GLUT1 densities reflect local glucose metabolism in the brain, we conclude that local MCT1 densities are adjusted to local glucose metabolism and transport.
单羧酸转运蛋白1（MCT1）促进单羧酸穿过血脑屏障和脑实质细胞的细胞膜。本研究有两个目的：（1）确定MCT1在脑中的局部分布；（2）比较MCT1的局部密度与主要营养转运蛋白葡萄糖转运蛋白GLUT1的局部密度。使用大鼠脑冰冻切片的免疫放射自显影法，分析了32个脑结构。（1）在整个脑中观察到MCT1密度的异质分布模式。与脑匀浆（100%）相比，在所研究的脑结构中，MCT1密度范围为43%至164%。局部GLUT1密度显示出相当的范围（35% - 145%）。（2）发现局部MCT1密度与局部GLUT1密度之间存在密切相关性。由于局部GLUT1密度反映了脑中的局部葡萄糖代谢，我们得出结论，局部MCT1密度是根据局部葡萄糖代谢和转运进行调节的。
