Su Zhi-Hui, Imura Yûki, Okamoto Munehiro, Osawa Syozo
JT Biohistory Research Hall, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan.
Gene. 2004 Feb 4;326:43-57. doi: 10.1016/j.gene.2003.10.025.
The phylogenetic position of the tribe Cychrini within the subfamily Carabinae (the family Carabidae) was estimated by comparing the nucleotide sequences of the mitochondrial NADH dehydrogenase subunit 5 (ND5) gene and the nuclear 28S ribosomal DNA (rDNA). The phylogenetic trees suggest that the Cychrini would most probably be the oldest line within the Carabinae. Phylogenetic trees were constructed by comparing the mitochondrial cytochrome C oxidase subunit I (COI) gene sequences from 33 species of the Cychrini from various localities that include the whole distribution ranges of the representative species within all the known genera in the world. The trees suggest that the Cychrini members radiated into a number of phylogenetic lineages within a short period, starting about 44 million years ago (MYA). Most of the phylogenetic lineages or sublineages are geographically linked, each consisting of a single or only a few species without scarce morphological differentiation in spite of their long evolutionary histories (silent or near-silent evolution [see Adv. Biophys. 36 (1999) 65; J. Mol. Evol. 53 (2001) 517]). The fact suggests that the geographic isolation per se did not bring about conspicuous morphological differentiation. The phylogenetic lineages of the Cychrini well correspond to the taxonomically defined genera and the subgenera.
通过比较线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基5（ND5）基因和核28S核糖体DNA（rDNA）的核苷酸序列，估算了扁甲亚科（步甲科）中扁甲族的系统发育位置。系统发育树表明，扁甲族很可能是扁甲亚科中最古老的谱系。通过比较来自世界各地的33种扁甲族物种的线粒体细胞色素C氧化酶亚基I（COI）基因序列构建了系统发育树，这些物种涵盖了世界上所有已知属中代表性物种的整个分布范围。这些树表明，扁甲族成员在大约4400万年前开始的短时间内辐射形成了许多系统发育谱系。大多数系统发育谱系或亚谱系在地理上相互关联，每个谱系由一个或仅几个物种组成，尽管它们有着漫长的进化历史（沉默或近乎沉默的进化[见《生物物理学进展》36（1999）65；《分子进化杂志》53（2001）517]），但形态分化并不明显。这一事实表明，地理隔离本身并没有导致明显的形态分化。扁甲族的系统发育谱系与分类学上定义的属和亚属非常吻合。
