体内雌激素预处理对大鼠离体逼尿肌收缩反应的影响。

The effect of in vivo oestrogen pretreatment on the contractile response of rat isolated detrusor muscle.

作者信息

Elliott R A, Castleden C M, Miodrag A

机构信息

Department of Medicine for the Elderly, Leicester General Hospital.

出版信息

Br J Pharmacol. 1992 Nov;107(3):766-70. doi: 10.1111/j.1476-5381.1992.tb14521.x.

DOI:10.1111/j.1476-5381.1992.tb14521.x

PMID:1472973

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1907756/

Abstract

The effect of oestradiol pretreatment was investigated on the response of rat isolated detrusor muscle to cholinergic, electrical and 5-hydroxytryptamine (5-HT) stimulation with and without diethylstilbestrol (DES) (2 microM) in the organ bath. 2. Virgin female Wistar rats were injected subcutaneously for 8 days with oestradiol benzoate 150 micrograms kg-1. Control rats received no injections or injection only with the vehicle, ethyl oleate. 3. Detrusor muscle from treated rats showed a decreased sensitivity to acetylcholine (ACh) and carbachol-induced contractile responses. The dose-response curves to these agonists showed a 44% reduction in maximum contractile response for ACh (P < 0.001), and a 38% reduction in maximum contractile response for carbachol (P < 0.05). The addition of 2 microM DES to the bathing medium further significantly reduced the maximum contractile response by 56 and 57% of control respectively. 4. Electrically stimulated detrusor muscle from treated rats showed a significant 49% reduction in the maximum contractile response (P < 0.001). The addition of 2 microM DES to the bathing medium further significantly reduced the maximum contractile response by 66% of control. The tetrodotoxin resistant responses were smaller in pretreated rats, suggesting a reduced sensitivity of the smooth muscle to direct electrical stimulation. 5. The response to 5-HT stimulation by detrusor muscle samples from oestradiol-treated rats showed a non-significant reduction in maximum contractile response, but the addition of 2 microM DES to the bath chamber resulted in a 67% reduction in the response (P < 0.001). 6. Oestradiol pretreatment did not affect the potassium dose-response curve.7. Oestradiol pretreatment reduced the rat detrusor muscle sensitivity to the blocking effect of atropine on the response to electrical field stimulation. Pretreatment also reduced the potentiating effect of physostigmine on the same response.8. These results suggest that oestradiol pretreatment had a modulating effect on cholinergic responses.The addition of oestrogen to the tissue environment enhances this inhibitory effect.

摘要

在器官浴槽中，研究了雌二醇预处理对大鼠离体逼尿肌对胆碱能、电刺激和5-羟色胺（5-HT）刺激的反应的影响，刺激条件为有无己烯雌酚（DES）（2微摩尔）。2. 成年雌性Wistar大鼠皮下注射苯甲酸雌二醇150微克/千克，持续8天。对照大鼠不注射或仅注射溶剂油酸乙酯。3. 处理组大鼠的逼尿肌对乙酰胆碱（ACh）和卡巴胆碱诱导的收缩反应敏感性降低。这些激动剂的剂量-反应曲线显示，ACh的最大收缩反应降低了44%（P<0.001），卡巴胆碱的最大收缩反应降低了38%（P<0.05）。向浴槽中添加2微摩尔DES进一步显著降低了最大收缩反应，分别降至对照的56%和57%。4. 处理组大鼠经电刺激的逼尿肌最大收缩反应显著降低了49%（P<0.001）。向浴槽中添加2微摩尔DES进一步显著降低了最大收缩反应，降至对照的66%。预处理大鼠中对河豚毒素耐受的反应较小，表明平滑肌对直接电刺激的敏感性降低。5. 雌二醇处理组大鼠逼尿肌样本对5-HT刺激的反应最大收缩反应无显著降低，但向浴槽中添加2微摩尔DES导致反应降低了67%（P<0.001）。6. 雌二醇预处理不影响钾剂量-反应曲线。7. 雌二醇预处理降低了大鼠逼尿肌对阿托品对电场刺激反应的阻断作用的敏感性。预处理还降低了毒扁豆碱对相同反应的增强作用。8. 这些结果表明，雌二醇预处理对胆碱能反应有调节作用。向组织环境中添加雌激素可增强这种抑制作用。