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用于脂蛋白电泳分离的非变性复合梯度凝胶制备的改进方法。

Improved method for making nondenaturing composite gradient gels for the electrophoretic separation of lipoproteins.

作者信息

Rainwater David L, Moore Perry H, Gamboa Israel O

机构信息

Department of Genetics, Southwest Foundation for Biomedical Research, PO Box 760549, San Antonio, TX 78245-0549, USA.

出版信息

J Lipid Res. 2004 Apr;45(4):773-5. doi: 10.1194/jlr.D300035-JLR200. Epub 2004 Jan 16.

DOI:10.1194/jlr.D300035-JLR200
PMID:14729853
Abstract

Nondenaturing gradient gel electrophoresis continues to be used widely for resolution and characterization of lipoprotein subclasses. Methods for making such gels in the laboratory have been published, but occasionally samples do not display uniform mobilities for all lanes in a laboratory-made gel. To help overcome this limitation, we recommend a modification

摘要

非变性梯度凝胶电泳仍被广泛用于脂蛋白亚类的分离和鉴定。实验室制备此类凝胶的方法已有报道,但有时在实验室自制的凝胶中,样品在所有泳道中的迁移率并不一致。为帮助克服这一局限性,我们推荐一种改进方法

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