López-Cepero José M
Department of Cell Biology, Histology and Pathology, Faculty of Medicine, University of Cádiz, Spain.
J Histochem Cytochem. 2004 Feb;52(2):211-6. doi: 10.1177/002215540405200209.
Silver staining methods, when selective, yield a high-contrast and high-resolution image in optical microscopy. A classical method for silver impregnation of mitochondria has been applied to murine tissues and reveals a marked heterogeneity among mitochondria in single cells. This heterogeneity can be detected in the optical microscope but is even more evident at the ultrastructural level. The differences in staining intensity may reflect different stages in the mitochondrial life cycle. The progressive accumulation of uranyl-argyrophilic material may be a marker of mitochondrial aging. This highly selective staining procedure may be of use in studies of mitochondrial changes under pathological conditions and during apoptosis.
银染方法在具有选择性时，能在光学显微镜下产生高对比度和高分辨率的图像。一种经典的线粒体银浸染方法已应用于小鼠组织，揭示了单细胞中线粒体之间存在明显的异质性。这种异质性在光学显微镜下即可检测到，但在超微结构水平上更为明显。染色强度的差异可能反映了线粒体生命周期的不同阶段。铀酰嗜银物质的逐渐积累可能是线粒体衰老的一个标志。这种高度选择性的染色程序可能有助于研究病理条件下和细胞凋亡过程中的线粒体变化。
