Ramaswamy Srinivas V, Dou Shu-Jun, Rendon Adrian, Yang Zhenhua, Cave M Donald, Graviss Edward A
Houston Tuberculosis Initiative, Department of Pathology, Baylor College of Medicine, Houston, TX, USA 2Pulmonary Services and Clinical Pathology Laboratory, University Hospital of Monterrey, Universidad Autonomy de Nuevo Leon, Nuevo Leon, Mexico 3Regional Tuberculosis Genotyping Laboratory, Central Arkansas Veterans Healthcare System, AR, USA 4Department of Medicine and 5Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
J Med Microbiol. 2004 Feb;53(Pt 2):107-113. doi: 10.1099/jmm.0.05343-0.
Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RFLP patterns were obtained from the multidrug-resistant tuberculosis (MDR-TB) isolates. Approximately 65% of the MDR-TB isolates were attributed to secondary resistance. Different drug-susceptibility patterns were seen with the clustered isolates. The percentage of isolates resistant to isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and streptomycin (STR) was 100, 97.3, 48.7 and 67.6, respectively. The most common resistance-associated polymorphisms for the four drugs were as follows: INH, Ser315Thr (67.6%) in katG; RIF, Ser450Leu (41.7%) in rpoB; EMB, Met306Ile/Val/Leu (66.7%) in embB; and STR, Lys43Arg (24%) in rpsL. Drug-resistance-associated mutations were similar to changes occurring in isolates from other areas of the world, but unique, previously unreported, mutations in katG (n=5), rpoB (n=1) and rrs (n=3) were also identified.
对37株耐多药结核分枝杆菌和13株全敏感结核分枝杆菌进行分析，以研究与已知耐药机制相关的基因型多样性。这些菌株来自墨西哥蒙特雷一所大学结核病诊所的患者。从耐多药结核病（MDR-TB）菌株中总共获得了25种IS6110-RFLP模式。约65%的MDR-TB菌株归因于继发性耐药。聚类菌株呈现出不同的药敏模式。对异烟肼（INH）、利福平（RIF）、乙胺丁醇（EMB）和链霉素（STR）耐药的菌株百分比分别为100%、97.3%、48.7%和67.6%。这四种药物最常见的耐药相关多态性如下：INH，katG基因中的Ser315Thr（67.6%）；RIF，rpoB基因中的Ser450Leu（41.7%）；EMB，embB基因中的Met306Ile/Val/Leu（66.7%）；STR，rpsL基因中的Lys43Arg（24%）。耐药相关突变与世界其他地区分离株中发生的变化相似，但也鉴定出了katG（n = 5）、rpoB（n = 1）和rrs（n = 3）中独特的、先前未报道的突变。
