酿酒酵母TIF4631基因推定的内部核糖体进入位点中启动子的定位
Localization of a promoter in the putative internal ribosome entry site of the Saccharomyces cerevisiae TIF4631 gene.
作者信息
Vergé Valérie, Vonlanthen Martin, Masson Jean-Michel, Trachsel Hans, Altmann Michael
机构信息
Institute for Biochemistry and Molecular Biology, University of Berne, CH-3012 Bern, Switzerland.
出版信息
RNA. 2004 Feb;10(2):277-86. doi: 10.1261/rna.5910104.
Abstract
The 5'-region of the TIF4631 gene of Saccharomyces cerevisiae (encoding the translation initiation factor eIF4G1) was reported earlier to harbor a very active internal ribosome entry site (IRES) allowing for internal initiation of translation of TIF4631 mRNA. Here, we report the presence of a promoter in the region -112 to -36 relative to the translation initiation codon of the TIF4631 gene. This promoter stimulates transcription from a start site at position -36 and generates an mRNA that is actively translated in vitro and able to sustain growth of yeast cells in vivo as the only source of eIF4G. The data show that the IRES activity reported earlier is due to this promoter. On the contrary, the presumed IRES represents a strongly inhibitory element for translation in vitro.
摘要
早前有报道称，酿酒酵母的TIF4631基因（编码翻译起始因子eIF4G1）的5'区域含有一个非常活跃的内部核糖体进入位点（IRES），可实现TIF4631 mRNA的内部翻译起始。在此，我们报告在相对于TIF4631基因翻译起始密码子的-112至-36区域存在一个启动子。该启动子刺激从-36位的起始位点开始转录，并产生一种mRNA，该mRNA在体外能被积极翻译，并且在体内作为eIF4G的唯一来源能够维持酵母细胞的生长。数据表明，早前报道的IRES活性是由于该启动子所致。相反，推测的IRES在体外翻译中是一个强烈的抑制元件。
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