翻译起始因子eIF4G介导体外多聚腺苷酸尾依赖性翻译。
Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation.
作者信息
Tarun S Z, Wells S E, Deardorff J A, Sachs A B
机构信息
Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9046-51. doi: 10.1073/pnas.94.17.9046.
Abstract
The yeast translation factor eIF4G associates with both the cap-binding protein eIF4E and the poly(A)-binding protein Pab1p. Here we report that the two yeast eIF4G homologs, Tif4631p and Tif4632p, share a conserved Pab1p-binding site. This site is required for Pab1p and poly(A) tails to stimulate the in vitro translation of uncapped polyadenylylated mRNA, and the region encompassing it is required for the cap and the poly(A) tail to synergistically stimulate translation. This region on Tif4631p becomes essential for cell growth when the eIF4E binding site on Tif4631p is mutated. Pab1p mutations also show synthetic lethal interactions with eIF4E mutations. These data suggest that eIF4G mediates poly(A) tail stimulated translation in vitro, and that Pab1p and the domain encompassing the Pab1p-binding site on eIF4G can compensate for partial loss of eIF4E function in vivo.
摘要
酵母翻译因子eIF4G与帽结合蛋白eIF4E和聚腺苷酸结合蛋白Pab1p均相关联。在此我们报道，酵母的两个eIF4G同源物Tif4631p和Tif4632p共享一个保守的Pab1p结合位点。该位点是Pab1p和聚腺苷酸尾刺激无帽多聚腺苷酸化mRNA体外翻译所必需的，且包含该位点的区域是帽和聚腺苷酸尾协同刺激翻译所必需的。当Tif4631p上的eIF4E结合位点发生突变时，Tif4631p上的该区域对细胞生长变得至关重要。Pab1p突变也显示出与eIF4E突变的合成致死相互作用。这些数据表明，eIF4G在体外介导聚腺苷酸尾刺激的翻译，并且Pab1p以及eIF4G上包含Pab1p结合位点的结构域在体内可以补偿eIF4E功能的部分丧失。
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