Doron D A, McCarron R M, Heldman E, Sirén A L, Spatz M, Feuerstein G, Pollard H B, Hallenbeck J M
Department of Neurology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Brain Res. 1992 Dec 4;597(2):346-9. doi: 10.1016/0006-8993(92)91493-x.
The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca(2+)-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.
在用不同剂量的脂多糖（LPS）、凝血酶、佛波醇肉豆蔻酸酯（PMA）、钙离子载体（A23187）或肿瘤坏死因子（TNF）和白细胞介素-1（IL-1）刺激来自正常血压的Wistar-Kyoto大鼠（WKY）和自发性高血压大鼠（SHR）的脑微血管内皮细胞（BMECs）后，比较了它们所表达的组织因子（TF）的量。用所有这些因子处理来自WKY和SHR的培养BMECs后，它们的TF总量呈剂量依赖性增加；在WKY和SHR的BMECs之间未观察到TF表达增强水平的实质性差异。我们得出结论，与正常血压对照组相比，高血压大鼠（主要的中风危险因素）受刺激的内皮细胞在TF表达方面并非反应过度。
