促肾上腺皮质激素释放因子（CRF）而非神经肽Y（NPY）通过N-甲基-D-天冬氨酸（NMDA）受体参与焦虑调节。

Involvement of CRF but not NPY in the anxiety regulation via NMDA receptors.

作者信息

Wierońska Joanna M, Szewczyk Bernadeta, Pałucha Agnieszka, Brański Piotr, Smiałowska Maria

机构信息

Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, PL 31-343 Kraków, Poland.

出版信息

Pol J Pharmacol. 2003 Nov-Dec;55(6):1119-24.

PMID:14730109

Abstract

The study attempts to evaluate whether neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) are involved in anxiogenic and anxiolytic reactions induced by NMDA receptor ligands. The animals were given MK-801 (1 mg/kg, ip), a non-competitive NMDA-receptor antagonist, which acts as anxiolytic agent, or NMDA (15 mg/kg, ip), which has an anxiogenic effect. The anxiogenic or anxiolytic actions of these compounds were evaluated in the plus-maze test. The animals, which were given MK-801, were administered BIBO 3304 (130 ng/0.5 microl/site) intraamygdalarly and the animals which were given NMDA were administered alpha-helical CRF (500 ng/0.5 microl/site). BIBO 3304 did not attenuate MK-801-induced anxiolysis and alpha-helical CRF abolished NMDA-induced anxiogenesis. Our results show that anxiogenic effect of NMDA is mediated via CRF1 receptors and anxiolytic action of MK-801 is not dependent on Y1 receptors.

摘要

该研究试图评估神经肽Y（NPY）和促肾上腺皮质激素释放因子（CRF）是否参与N-甲基-D-天冬氨酸（NMDA）受体配体诱导的致焦虑和抗焦虑反应。给动物注射作为抗焦虑剂的非竞争性NMDA受体拮抗剂MK-801（1毫克/千克，腹腔注射），或具有致焦虑作用的NMDA（15毫克/千克，腹腔注射）。在十字迷宫试验中评估这些化合物的致焦虑或抗焦虑作用。给注射MK-801的动物杏仁核内注射BIBO 3304（130纳克/0.5微升/部位），给注射NMDA的动物杏仁核内注射α-螺旋CRF（500纳克/0.5微升/部位）。BIBO 3304未减弱MK-801诱导的抗焦虑作用，而α-螺旋CRF消除了NMDA诱导的致焦虑作用。我们的结果表明，NMDA的致焦虑作用是通过CRF1受体介导的，而MK-801的抗焦虑作用不依赖于Y1受体。