Wierońska Joanna M, Szewczyk Bernadeta, Pałucha Agnieszka, Brański Piotr, Smiałowska Maria
Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, PL 31-343 Kraków, Poland.
Pol J Pharmacol. 2003 Nov-Dec;55(6):1119-24.
The study attempts to evaluate whether neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) are involved in anxiogenic and anxiolytic reactions induced by NMDA receptor ligands. The animals were given MK-801 (1 mg/kg, ip), a non-competitive NMDA-receptor antagonist, which acts as anxiolytic agent, or NMDA (15 mg/kg, ip), which has an anxiogenic effect. The anxiogenic or anxiolytic actions of these compounds were evaluated in the plus-maze test. The animals, which were given MK-801, were administered BIBO 3304 (130 ng/0.5 microl/site) intraamygdalarly and the animals which were given NMDA were administered alpha-helical CRF (500 ng/0.5 microl/site). BIBO 3304 did not attenuate MK-801-induced anxiolysis and alpha-helical CRF abolished NMDA-induced anxiogenesis. Our results show that anxiogenic effect of NMDA is mediated via CRF1 receptors and anxiolytic action of MK-801 is not dependent on Y1 receptors.
该研究试图评估神经肽Y（NPY）和促肾上腺皮质激素释放因子（CRF）是否参与N-甲基-D-天冬氨酸（NMDA）受体配体诱导的致焦虑和抗焦虑反应。给动物注射作为抗焦虑剂的非竞争性NMDA受体拮抗剂MK-801（1毫克/千克，腹腔注射），或具有致焦虑作用的NMDA（15毫克/千克，腹腔注射）。在十字迷宫试验中评估这些化合物的致焦虑或抗焦虑作用。给注射MK-801的动物杏仁核内注射BIBO 3304（130纳克/0.5微升/部位），给注射NMDA的动物杏仁核内注射α-螺旋CRF（500纳克/0.5微升/部位）。BIBO 3304未减弱MK-801诱导的抗焦虑作用，而α-螺旋CRF消除了NMDA诱导的致焦虑作用。我们的结果表明，NMDA的致焦虑作用是通过CRF1受体介导的，而MK-801的抗焦虑作用不依赖于Y1受体。
