Hierlihy L E, Wallace J L, Ferguson A V
Department of Physiology, Queen's University, Kingston, Ont., Canada.
Can J Physiol Pharmacol. 1992 Aug;70(8):1109-16. doi: 10.1139/y92-154.
The role of the pituitary hormones in the development of neurally mediated gastric mucosal damage was examined in both normal and hypophysectomized urethane-anaesthetized male Sprague-Dawley rats. Gastric mucosal damage was elicited either by electrical stimulation of intact vagal nerves or by electrical stimulation in the paraventricular nucleus. Macroscopic damage was scored following the stimulation period and samples of the stomach were fixed for histological assessment. Damage scores were assigned based on a 0 (normal) to 3 (severe) scale. Control experiments in which the vagi were not stimulated did not result in any significant gastric damage in either normal (0.56) or sham surgery (0.14) animals, whereas hypophysectomized animals were observed to have significant damage (1.44, p < 0.05). Stimulation of the vagi in hypophysectomized animals resulted in damage that was not significantly different compared with the hypophysectomized control animals (1.25, p > 0.05). In normal animals, stimulation of vagal nerves resulted in mean damage scores of 2.00, values that were not significantly different from those observed in hypophysectomized animals (1.25, p > 0.05). Similarly, stimulation in the paraventricular nucleus of hypophysectomized animals resulted in gastric lesions (2.00) that were not significantly different from those observed in normal animals (1.91, p > 0.05). These data suggest that such neurally mediated gastric damage does not depend upon neurosecretory projections to the pituitary gland, but that the maintenance of an intact gastric mucosa under normal conditions requires the presence of pituitary hormones.
在正常和垂体切除的氨基甲酸乙酯麻醉的雄性斯普拉格 - 道利大鼠中，研究了垂体激素在神经介导的胃黏膜损伤发展中的作用。通过完整迷走神经的电刺激或室旁核的电刺激引发胃黏膜损伤。刺激期后对宏观损伤进行评分，并将胃样本固定用于组织学评估。损伤评分基于0（正常）至3（严重）的量表进行分配。在未刺激迷走神经的对照实验中，正常动物（0.56）或假手术动物（0.14）均未出现任何明显的胃损伤，而观察到垂体切除的动物有明显损伤（1.44，p<0.05）。垂体切除动物中迷走神经的刺激导致的损伤与垂体切除对照动物相比无显著差异（1.25，p>0.05）。在正常动物中，迷走神经刺激导致的平均损伤评分为2.00，与垂体切除动物中观察到的值无显著差异（1.25，p>0.05）。同样，垂体切除动物室旁核的刺激导致的胃损伤（2.00）与正常动物中观察到的损伤无显著差异（1.91，p>0.05）。这些数据表明，这种神经介导的胃损伤不依赖于向垂体的神经分泌投射，但在正常情况下胃黏膜的维持需要垂体激素的存在。
