Kubán Pavel, Kubán Petr, Hauser Peter C, Kubán Vlastimil
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic.
Electrophoresis. 2004 Jan;25(1):35-42. doi: 10.1002/elps.200305674.
The system comprises two flow injection-capillary electrophoresis interfaces into which the opposite ends of the separation capillary are inserted. The electrolyte solution flows through both interfaces by use of hydrostatic pressure. The injection of the samples into the electrolyte flow is accomplished by a rotary-type chromatographic valve at the grounded side and by a pinch-valve injector at the high-voltage side that provides sufficient isolation from the high electric field. The system allows a fully automated dual-injection sequence of samples from both capillary ends and simultaneous electrophoretic separation of anions and cations in the samples. The analytes are detected by a high-voltage contactless conductometric detector positioned approximately in the middle of the separation capillary. The parameters of the system were evaluated. The repeatability of the flow injection-capillary electrophoresis system for the simultaneous determination of anions and cations was evaluated for ten consecutive injections and relative standard deviation (RSD) values for peak areas were better than 1.0%. The sample throughput for total ionic analysis was estimated to be 25 samples per hour. The system was used for automated simultaneous analysis of anions and cations in various real samples. Using a short separation capillary, rapid total ionic analysis in less then 1 min is demonstrated.
该系统由两个流动注射-毛细管电泳接口组成，分离毛细管的两端插入其中。电解液通过静水压流经两个接口。样品通过接地侧的旋转式色谱阀和高压侧的夹管阀进样器注入电解液流中，夹管阀进样器可提供与高电场的充分隔离。该系统允许从毛细管两端对样品进行全自动双进样，并同时对样品中的阴离子和阳离子进行电泳分离。分析物由位于分离毛细管大约中间位置的高压非接触式电导检测器检测。对该系统的参数进行了评估。对流动注射-毛细管电泳系统同时测定阴离子和阳离子的重复性进行了评估，连续进样十次，峰面积的相对标准偏差（RSD）值优于1.0%。总离子分析的样品通量估计为每小时25个样品。该系统用于各种实际样品中阴离子和阳离子的自动同时分析。使用短分离毛细管，可在不到1分钟内实现快速总离子分析。
