Simonet Bartolomé M, Ríos Angel, Valcárcel Miguel
Analytical Chemistry Department, University of Córdoba, Córdoba, Spain.
Electrophoresis. 2004 Jan;25(1):50-6. doi: 10.1002/elps.200305653.
Enzymes immobilized on the inner surface of an electrophoretic capillary were used to increase sensitivity and resolution in capillary zone electrophoresis (CZE). Sensitivity is enhanced by inserting a piece of capillary containing the immobilized enzyme into the main capillary, located before the detector, in order to transform the analyte into a product with a higher absorptivity. This approach was used to determine ethanol. In order to improve resolution, capillary pieces containing immobilized enzymes were inserted at various strategic positions along the electrophoretic capillary. On reaching the enzyme, the analyte was converted into a product with a high electrophoretic mobility, the migration time for which was a function of the position of the enzyme reactor. This approach was applied to the separation and determination of acetaldehyde and pyruvate. Finally, the proposed method was validated with the determination of ethanol, acetaldehyde, and pyruvate in beer and wine samples.
固定在电泳毛细管内表面的酶被用于提高毛细管区带电泳（CZE）的灵敏度和分辨率。通过将一段装有固定化酶的毛细管插入位于检测器之前的主毛细管中，将分析物转化为具有更高吸光度的产物，从而提高灵敏度。该方法用于测定乙醇。为了提高分辨率，将装有固定化酶的毛细管片段沿电泳毛细管的不同关键位置插入。分析物到达酶时，会转化为具有高电泳迁移率的产物，其迁移时间是酶反应器位置的函数。该方法应用于乙醛和丙酮酸的分离与测定。最后，通过测定啤酒和葡萄酒样品中的乙醇、乙醛和丙酮酸对所提出的方法进行了验证。
