Lu Qin, Collins Greg E, Evans Thomas, Hammond Mark, Wang Joseph, Mulchandani Ashok
Geo Centers, Inc., Lanham, MD, USA.
Electrophoresis. 2004 Jan;25(1):116-22. doi: 10.1002/elps.200305710.
A capillary electrophoresis microchip is used to selectively and sensitively monitor cyanide levels in both vapor (HCN((g))) and aqueous (NaCN in drinking water) phases. Laser-induced fluorescence detection is applied using a violet diode laser to monitor the fluorescent isoindole derivative formed by the reaction of cyanide with 2,3-naphthalenedicarboxaldehyde (NDA) and taurine. Air sampling of hydrogen cyanide is achieved using a miniature impinger (2 mL), giving collection efficiencies as high as 79% for a sampling rate of 1.0 L/min and a 10 s sampling time (relative standard deviation RSD: 2.7% for n = 5). Following the addition of NDA and taurine to either the vapor phase impinger sample or an aqueous drinking water sample, the NDA/cyanide derivative can be detected in just over 40 s on the microchip, giving a detection limit of 0.56 microg/L and a linear dynamic range from 0.56 microg/L-2.4 mg/L. The detection limit for hydrogen cyanide in air was determined to be 2.3 ppb (mole%). On-chip derivatization of cyanide by NDA was successful, although a 50% decrease in signal intensity was observed due to insufficient time for completion of the reaction on the microchip. A number of different interferents were examined, and only iron(II) and chlorine showed any interference due to their capability for masking the presence of cyanide by reacting with free cyanide in solution.
一种毛细管电泳微芯片用于选择性和灵敏地监测气相（HCN（g））和水相（饮用水中的NaCN）中的氰化物水平。使用紫光二极管激光器进行激光诱导荧光检测，以监测氰化物与2,3-萘二甲醛（NDA）和牛磺酸反应形成的荧光异吲哚衍生物。使用微型冲击器（2 mL）对氰化氢进行空气采样，对于1.0 L/min的采样速率和10 s的采样时间，收集效率高达79%（n = 5时相对标准偏差RSD：2.7%）。在向气相冲击器样品或饮用水水相样品中加入NDA和牛磺酸后，微芯片上仅需40多秒就能检测到NDA/氰化物衍生物，检测限为0.56 μg/L，线性动态范围为0.56 μg/L - 2.4 mg/L。空气中氰化氢的检测限确定为2.3 ppb（摩尔%）。尽管由于微芯片上反应时间不足导致信号强度下降了50%，但NDA对氰化物的芯片上衍生化是成功的。研究了多种不同的干扰物，只有铁（II）和氯表现出干扰，因为它们能够通过与溶液中的游离氰化物反应来掩盖氰化物的存在。
