新生霉素抑制血管生成并与长春新碱显示协同效应。

[Novobiocin inhibits angiogenesis and shows synergistic effect with vincristine].

作者信息

Yang Jun, Jiang Min, Zhen Yong-su

机构信息

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.

出版信息

Yao Xue Xue Bao. 2003 Oct;38(10):731-4.

PMID:14730893

Abstract

AIM

To study the anti-angiogenic activity of novobiocin and its mechanism of action.

METHODS

The anti-angiogenic activity of novobiocin was determined using chick embryo chorioallantoic membrane(CAM) assay. MTT assay, zymography and related assays were used to observe the effects of drugs on bovine aorta endothelial cells and human pulmonary carcinoma PG cells.

RESULTS

Novobiocin at the doses of 100 and 200 micrograms/egg inhibited angiogenesis by 31.6% and 68.7% in CAM, respectively. The combination of novobiocin and vincristine enhanced the anti-angiogenic effect. Novobiocin inhibited the proliferation of bovine aortic endothelial cells in a concentration-dependent manner. In addition, novobiocin suppressed MMP-2 secretion, migration, and tube formation of endothelial cells. As determined by MTT assay, novobiocin in combination with vincristine displayed synergistic effect on the proliferation of PG cells,

CONCLUSION

This study demonstrates that novobiocin is active in suppressing angiogenesis and the anti-angiogenic activity may be enhanced by combination with vincristine. The anti-angiogenic activity of novobiocin may be related, at least in part, to its inhibition of cell proliferation, cell migration, tube formation and secretion of matrix metalloproteinases.

摘要

目的

研究新生霉素的抗血管生成活性及其作用机制。

方法

采用鸡胚绒毛尿囊膜（CAM）试验测定新生霉素的抗血管生成活性。采用MTT法、酶谱法及相关试验观察药物对牛主动脉内皮细胞和人肺癌PG细胞的影响。

结果

新生霉素剂量为100和200微克/枚鸡蛋时，分别抑制CAM血管生成31.6%和68.7%。新生霉素与长春新碱联合使用增强了抗血管生成作用。新生霉素以浓度依赖方式抑制牛主动脉内皮细胞的增殖。此外，新生霉素抑制内皮细胞MMP-2分泌、迁移和管腔形成。MTT法测定结果显示，新生霉素与长春新碱联合使用对PG细胞增殖具有协同作用。