利福平与氟喹诺酮类药物对人巨噬细胞内结核杆菌的作用。

The effects of rifampicin and fluoroquinolones on tubercle bacilli within human macrophages.

作者信息

Duman Necdet, Cevikbaş Adile, Johansson Candan

机构信息

Department of Microbiology and Clinical Microbiology, Faculty of Medicine University of Marmara, Haydarpaşa, Istanbul, Turkey.

出版信息

Int J Antimicrob Agents. 2004 Jan;23(1):84-7. doi: 10.1016/j.ijantimicag.2003.05.017.

DOI:10.1016/j.ijantimicag.2003.05.017

PMID:14732319

Abstract

The objective of this study was to determine the activities of rifampicin, ciprofloxacin and sparfloxacin against M. tuberculosis H37Rv in human macrophages. The minimal inhibitory concentrations (MIC) were determined by agar macrodilution and broth microdilution methods and were 0.5, 1 and 0.125 mg/l, respectively. Concentrations of rifampicin at 0.5 and 2.5 microg (P<0.001), ciprofloxacin at 4 and 8 microg (P<0.001) and sparfloxacin at 0.125 microg (P<0.05), 0.625 microg (P<0.001) and 1.25 microg (P<0.001) were found to be effective against intracellular bacteria. Ciprofloxacin and especially sparfloxacin were effective in macrophages and may be useful in the treatment of tuberculosis particularly infections caused by multiply drug resistant strains.

摘要

本研究的目的是测定利福平、环丙沙星和司帕沙星对人巨噬细胞内结核分枝杆菌H37Rv的活性。通过琼脂大稀释法和肉汤微量稀释法测定最低抑菌浓度（MIC），分别为0.5、1和0.125mg/l。发现利福平浓度为0.5和2.5μg（P<0.001）、环丙沙星浓度为4和8μg（P<0.001）以及司帕沙星浓度为0.125μg（P<0.05）、0.625μg（P<0.001）和1.25μg（P<0.001）对细胞内细菌有效。环丙沙星尤其是司帕沙星在巨噬细胞中有效，可能对治疗结核病特别是多重耐药菌株引起的感染有用。