趋化因子受体CXCR4、CCR2、CCR5和CX3CR1在从成年大鼠脑室下区分离出的神经祖细胞中的表达。

Expression of chemokine receptors CXCR4, CCR2, CCR5 and CX3CR1 in neural progenitor cells isolated from the subventricular zone of the adult rat brain.

作者信息

Ji Jun Feng, He Bei Ping, Dheen S Thameem, Tay Samuel Sam Wah

机构信息

Department of Anatomy, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 117597, Singapore.

出版信息

Neurosci Lett. 2004 Jan 30;355(3):236-40. doi: 10.1016/j.neulet.2003.11.024.

DOI:10.1016/j.neulet.2003.11.024

PMID:14732474

Abstract

We have studied the expression of chemokine receptors CXCR4, CCR2, CCR5, and CX3CR1 at the mRNA and protein levels in adult neural progenitor cells (NPCs) in neurosphere cultures using RT-PCR and immunocytochemistry methods. NPCs were isolated from the subventricular zone of adult rat brain and propagated in vitro as neurospheres. The neurospheres showed immunoactivity of nestin, an intermediate filament marker for NPCs. NPCs in the neurosphere cultures differentiated into NeuN-, GFAP-, or GalC-positive cells in vitro. Using cultured cortical microglial cells as positive control, we demonstrated the mRNA expression of CXCR4, CCR2, CCR5, and CX3CR1 in neurospheres by RT-PCR. Double immunofluorescent staining further confirmed the co-localization of nestin with either CXCR4, CCR2, CCR5, or CX3CR1 on neurospheres. These results suggest that adult NPCs in the neurosphere cultures express chemokine receptors CXCR4, CCR2, CCR5, and CX3CR1.

摘要

我们使用逆转录聚合酶链反应（RT-PCR）和免疫细胞化学方法，研究了神经球培养物中成年神经祖细胞（NPCs）在mRNA和蛋白质水平上趋化因子受体CXCR4、CCR2、CCR5和CX3CR1的表达。从成年大鼠脑的脑室下区分离出NPCs，并在体外作为神经球进行增殖培养。这些神经球显示出巢蛋白的免疫活性，巢蛋白是NPCs的一种中间丝标志物。神经球培养物中的NPCs在体外分化为NeuN、GFAP或GalC阳性细胞。以培养的皮质小胶质细胞作为阳性对照，我们通过RT-PCR证明了神经球中CXCR4、CCR2、CCR5和CX3CR1的mRNA表达。双重免疫荧光染色进一步证实了巢蛋白与CXCR4、CCR2、CCR5或CX3CR1在神经球上的共定位。这些结果表明，神经球培养物中的成年NPCs表达趋化因子受体CXCR4、CCR2、CCR5和CX3CR1。