Jiang Y, Salafranca M N, Adhikari S, Xia Y, Feng L, Sonntag M K, deFiebre C M, Pennell N A, Streit W J, Harrison J K
Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville 32610-0267, USA.
J Neuroimmunol. 1998 Jun 1;86(1):1-12. doi: 10.1016/s0165-5728(98)00005-8.
Chemokines are a group of pro-inflammatory peptides that mediate leukocyte migration and activation. Several members of the chemokine family have been shown to be synthesized by cells of the central nervous system (CNS). To begin to address the role of chemokine receptors in CNS physiology, we identified, by molecular cloning techniques, the rat orthologs of the chemokine receptors, CCR2, CCR3, CCR5, and CXCR4. CCR2 and CCR5 expression was detected in rat spleen, lung, kidney, thymus and macrophages; CCR5 mRNA was also detected in rat brain. Primary cultures of rat microglia expressed CCR5 mRNA that was regulated by IFN-gamma, while both cultured astrocytes and microglia were found to contain mRNA for CXCR4 and CX3CR1. Induction of experimental allergic encephalomyelitis (EAE) in the rat was accompanied by increased levels of CCR2, CCR5, CXCR4, and CX3CR1 mRNAs in the lumbar spinal cords of animals displaying clinical signs of the disease. These data identify the rat orthologs of chemokine receptors and demonstrate that brain, spinal cord, and cultured glial cells express chemokine receptors that can be regulated both in vitro and in vivo.
趋化因子是一类促炎肽，可介导白细胞迁移和活化。趋化因子家族的几个成员已被证明可由中枢神经系统（CNS）的细胞合成。为了开始研究趋化因子受体在CNS生理学中的作用，我们通过分子克隆技术鉴定了趋化因子受体CCR2、CCR3、CCR5和CXCR4的大鼠直系同源物。在大鼠脾脏、肺、肾、胸腺和巨噬细胞中检测到CCR2和CCR5的表达；在大鼠脑中也检测到CCR5 mRNA。大鼠小胶质细胞原代培养物表达受IFN-γ调节的CCR5 mRNA，而培养的星形胶质细胞和小胶质细胞均被发现含有CXCR4和CX3CR1的mRNA。在出现该疾病临床症状的动物的腰脊髓中，实验性变应性脑脊髓炎（EAE）的诱导伴随着CCR2、CCR5、CXCR4和CX3CR1 mRNA水平的升高。这些数据鉴定了趋化因子受体的大鼠直系同源物，并证明脑、脊髓和培养的神经胶质细胞表达可在体外和体内受到调节的趋化因子受体。
