Higuchi Masayuki, Hanada Yoshifumi, Ishikawa Seiichi, Ueda Eiichi, Takahashi Masanori
Kitakyushu City Institute of Environmental Sciences: 1-2-1, Shinike, Tobata-ku, Kitakyushu, Fukuoka 804-0082, Japan.
Shokuhin Eiseigaku Zasshi. 2003 Oct;44(5):263-7. doi: 10.3358/shokueishi.44.263.
A simultaneous determination method of 7 N-methylcarbamate and 7 urea pesticides in agricultural products by liquid chromatography/mass spectrometry (LC/MS) has been developed. Under reversed-phase liquid chromatographic conditions, 14 pesticides were analyzed using electrospray ionization (ESI) with simultaneous acquisition of positive ions and negative ions. Fourteen pesticides were extracted with acetone, 10% NaCl solution was added, and the pesticides were re-extracted with dichloromethane. The extract was concentrated under reduced pressure, and dissolved in methanol. The detection limits of 14 pesticides ranged from 0.0012 to 0.0056 microgram/g. The recoveries of pesticides were from 36.5 to 112.5% [RSD (n = 3) ranged from 0.5 to 48.1%] for 4 agricultural products.
建立了一种用液相色谱/质谱（LC/MS）同时测定农产品中7种氨基甲酸甲酯类和7种脲类农药的方法。在反相液相色谱条件下，采用电喷雾电离（ESI）同时采集正离子和负离子对14种农药进行分析。用丙酮提取14种农药，加入10%氯化钠溶液，再用二氯甲烷进行萃取。萃取液减压浓缩后，用甲醇溶解。14种农药的检出限为0.0012～0.0056微克/克。4种农产品中农药的回收率为36.5%～112.5%[相对标准偏差（n = 3）为0.5%～48.1%]。
