秘鲁利马艾滋病病毒阳性患者中的隐孢子虫种类和基因型
Cryptosporidium species and genotypes in HIV-positive patients in Lima, Peru.
作者信息
Cama Vitaliano A, Bern Caryn, Sulaiman I M, Gilman Robert H, Ticona Eduardo, Vivar Aldo, Kawai Vivian, Vargas Daniel, Zhou Ling, Xiao Lihua
机构信息
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
出版信息
J Eukaryot Microbiol. 2003;50 Suppl:531-3. doi: 10.1111/j.1550-7408.2003.tb00620.x.
Cryptosporidium parasites from a cross-sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to determine the diversity of Cryptosporidium spp. in HIV-positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium-positive specimens from 300 patients using a small subunit rRNA-based PCR-RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV-positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.
对来自秘鲁利马两家国立医院的一项横断面研究中的隐孢子虫寄生虫进行基因特征分析，以确定艾滋病毒阳性人群中隐孢子虫种类的多样性。共有2672名患者参与了这项研究，并提供了13937份标本。通过显微镜检查在354名（13.3%）患者中检测到隐孢子虫卵囊。使用基于小亚基rRNA的PCR-RFLP工具对来自300名患者的951份隐孢子虫阳性标本进行分析，鉴定出6种基因型；人隐孢子虫是最常检测到的种类（67.5%），其次是火鸡隐孢子虫（12.6%）和微小隐孢子虫（11.3%）。还发现了犬隐孢子虫（4.0%）、猫隐孢子虫（3.3%）和猪基因型隐孢子虫（0.5%）。这些发现表明，人隐孢子虫是秘鲁艾滋病毒阳性人群中的主要种类，并且人兽共患隐孢子虫种类占这些患者隐孢子虫病的约30%。
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