具有免疫能力的婴儿作为耶氏肺孢子菌的人类储存宿主：通过非侵入性采样和线粒体大亚基rRNA基因的实时PCR进行快速筛查。
Immunocompetent infants as a human reservoir for Pneumocystis jirovecii: rapid screening by non-invasive sampling and real-time PCR at the mitochondrial large subunit rRNA gene.
作者信息
Totet Anne, Meliani Leila, Lacube Philippe, Pautard Jean Claude, Raccurt Christian, Roux Patricia, Nevez Gilles
机构信息
Department of Parasitology, Mycology and Travel Medicine, University Hospital, University of Picardy, 80054 Amiens, France, EU.
出版信息
J Eukaryot Microbiol. 2003;50 Suppl:668-9. doi: 10.1111/j.1550-7408.2003.tb00678.x.
We tested a real-time PCR assay targeting the Pneumocystis jirovecii mitochondrial large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non-immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested-PCR assay targeting the same locus. Because of its one-step procedure, and its sensitivity and rapidity, the real-time PCR assay is particularly suitable for screening individuals parasitized by P. jirovecii within large populations.
我们在240份来自非免疫抑制婴儿的存档鼻咽抽吸物上，检测了一种针对耶氏肺孢子菌线粒体大亚基rRNA基因的实时PCR检测方法。该检测方法的灵敏度似乎与针对同一基因座的传统巢式PCR检测方法相近。由于其实时一步法操作，以及其灵敏度和快速性，这种实时PCR检测方法特别适合于在大量人群中筛查耶氏肺孢子菌感染个体。
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