猪RN区域的高密度连锁图谱。
A high-density linkage map of the RN region in pigs.
作者信息
Looft C, Milan D, Jeon J T, Paul S, Reinsch N, Rogel-Gaillard C, Rey V, Amarger V, Robic A, Kalm E, Chardon P, Andersson L
机构信息
Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, 24098 Kiel, Germany.
出版信息
Genet Sel Evol. 2000 May-Jun;32(3):321-9. doi: 10.1186/1297-9686-32-3-321.
The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.
猪的RN基因座影响肌肉糖原含量和肉质。我们之前已将RN基因座定位到15号染色体上。本研究描述了位于RN区域的4个I类和4个II类标记多态性的鉴定。利用F-SSCP标记（荧光单链构象多态性）和微卫星标记对资源家系进行基因分型。随后的多点连锁分析揭示了FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906的顺序。该基因顺序与之前报道的同一区域的猪RH图谱一致。所描述的图谱将有助于RN基因的定位克隆。
Zotero 插件