单次β辐射对人眼球筋膜成纤维细胞伤口愈合行为的影响。

The effects of single doses of beta radiation on the wound healing behaviour of human Tenon's capsule fibroblasts.

作者信息

Constable P H, Crowston J G, Occleston N L, Khaw P T

机构信息

International Collaborative Wound Healing Research Group, Institute of Ophthalmology and Moorfields Eye Hospital, London, UK.

出版信息

Br J Ophthalmol. 2004 Feb;88(2):169-73. doi: 10.1136/bjo.2003.020388.

DOI:10.1136/bjo.2003.020388

PMID:14736764

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1772003/

Abstract

AIM

To determine the effects of single doses of beta radiation on the wound healing functions of human Tenon's capsule fibroblasts (hTf).

METHODS

hTf were grown in tissue culture and irradiated with beta radiation using a strontium 90 source. The effects of beta radiation on fibroblast migration was studied using microporous transwell membranes. The effects of radiation on fibroblast contraction was investigated using a fibroblast populated collagen gels model. Production of extracellular matrix molecules (collagen I, collagen III, and fibronectin) by monolayers of irradiated fibroblasts was quantified for 14 days following single doses of beta radiation.

RESULTS

Growth inhibiting doses of beta radiation did not inhibit fibroblast migration or contraction at any time point. Levels of soluble fibronectin from irradiated populations were significantly reduced after >500 cGy beta radiation. Collagen I and III levels were not reduced after any dose of radiation, and increased following treatment with 1000 cGy beta radiation.

CONCLUSIONS

Growth arresting doses of beta radiation have unique effects on the wound healing behaviour of human Tenon's capsule fibroblasts. There was no significant effect on cellular migration or contraction, but ECM production was altered. Fibronectin production was inhibited following higher radiation doses, and collagen I and III production increased after 1000 cGy. The effects of single doses of beta radiation on ocular fibroblast wound healing behaviour are very different from those of 5-fluorouracil and mitomycin C, and these differences may be exploited clinically in the regulation of wound healing after glaucoma filtration surgery.

摘要

目的

确定单次β辐射剂量对人眼Tenon囊成纤维细胞（hTf）伤口愈合功能的影响。

方法

hTf在组织培养中生长，并用锶90源进行β辐射。使用微孔Transwell膜研究β辐射对成纤维细胞迁移的影响。使用成纤维细胞填充的胶原凝胶模型研究辐射对成纤维细胞收缩的影响。在单次β辐射剂量后的14天内，对受辐射的成纤维细胞单层产生的细胞外基质分子（I型胶原、III型胶原和纤连蛋白）进行定量。

结果

抑制生长剂量的β辐射在任何时间点均未抑制成纤维细胞的迁移或收缩。在>500 cGy的β辐射后，受辐射群体的可溶性纤连蛋白水平显著降低。任何剂量的辐射后，I型和III型胶原水平均未降低，而在接受1000 cGy辐射治疗后升高。

结论

抑制生长剂量的β辐射对人眼Tenon囊成纤维细胞的伤口愈合行为具有独特影响。对细胞迁移或收缩无显著影响，但细胞外基质的产生发生了改变。较高辐射剂量后纤连蛋白的产生受到抑制，而1000 cGy后I型和III型胶原的产生增加。单次β辐射剂量对眼内成纤维细胞伤口愈合行为的影响与5-氟尿嘧啶和丝裂霉素C的影响非常不同，这些差异可能在青光眼滤过手术后伤口愈合的临床调节中得到利用。