缺乏高亲和力白三烯B4受体BLT1的小鼠对内毒素诱导的葡萄膜炎的易感性并未降低。
Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B4 receptor.
作者信息
Smith J R, Subbarao K, Franc D T, Haribabu B, Rosenbaum J T
机构信息
Casey Eye Institute, Oregon Health & Science University, 3375 SW Terwilliger Boulevard, Portland, OR, USA.
出版信息
Br J Ophthalmol. 2004 Feb;88(2):273-5. doi: 10.1136/bjo.2003.027243.
AIM
To investigate the role of arachidonic acid derived chemotactic factor, LTB(4), in the development of endotoxin induced uveitis (EIU), using mice deficient in the BLT1 gene which encodes the high affinity LTB(4) receptor.
METHODS
BLT1 gene deficient and wild type BALB/c mice were injected intravitreally with Escherichia coli 055:B5 lipopolysaccharide (250 ng/2 microl). Number of leukocytes invading the anterior chamber 24 hours later were counted on tissue cross sections.
RESULTS
In all mice, EIU was characterised by a polymorphonuclear and mononuclear cell infiltrate. Numbers of infiltrating cells did not differ significantly between control and BLT1 gene knockout mice.
CONCLUSION
Chemotactic factors other than LTB(4) are primarily responsible for leukocyte migration into the eye during murine EIU.
目的
利用缺乏编码高亲和力白三烯B4（LTB4）受体的BLT1基因的小鼠，研究花生四烯酸衍生的趋化因子LTB4在内毒素诱导的葡萄膜炎（EIU）发病过程中的作用。
方法
向BLT1基因缺陷型和野生型BALB/c小鼠玻璃体内注射大肠杆菌055:B5脂多糖（250 ng/2微升）。24小时后，在组织切片上计数侵入前房的白细胞数量。
结果
在所有小鼠中，EIU的特征是多形核细胞和单核细胞浸润。对照小鼠和BLT1基因敲除小鼠之间浸润细胞的数量没有显著差异。
结论
在小鼠EIU期间，除LTB4外的趋化因子是白细胞迁移到眼内的主要原因。
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