Marín Bivens Carrie L, Grøndahl Christian, Murray Anthony, Blume Thorsten, Su You-Qiang, Eppig John J
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Biol Reprod. 2004 May;70(5):1458-64. doi: 10.1095/biolreprod.103.026351. Epub 2004 Jan 21.
The objective of this study was to determine the effects of a sterol found in ovarian follicular fluid, known as meiosis-activating sterol (FF-MAS), on the maturation of mouse oocytes in vitro. Possible effects of FF-MAS in promoting the metaphase I (MI) to metaphase II (MII) transition (nuclear maturation) and the competence of oocytes to complete preimplantation embryo development to the blastocyst stage (cytoplasmic maturation) were assessed. Cumulus cell-enclosed oocytes that were compromised in their ability to undergo nuclear maturation and subsequent development because of the age or genotype of the female were isolated at the germinal vesicle stage and matured in vitro using media supplemented with 0 to 20 microM FF-MAS. Oocytes that progressed to MII were inseminated in vitro, and the percentages developing to the 2-cell and blastocyst stages were determined. The sterol was omitted from the media used for oocyte insemination or preimplantation development. FF-MAS promoted a significantly higher percentage of oocytes in all groups to progress to MII in vitro. Moreover, FF-MAS treatment of oocytes maturing in vitro dramatically increased the competence of all but one of the groups of oocytes to complete preimplantation development. Therefore, FF-MAS improved mouse oocyte quality by promoting both nuclear and cytoplasmic maturation in vitro.
本研究的目的是确定在卵巢卵泡液中发现的一种固醇（减数分裂激活固醇，FF-MAS）对小鼠卵母细胞体外成熟的影响。评估了FF-MAS在促进中期I（MI）向中期II（MII）转变（核成熟）以及卵母细胞完成植入前胚胎发育至囊胚阶段的能力（细胞质成熟）方面的可能作用。由于雌性的年龄或基因型而在核成熟及后续发育能力上受损的卵丘细胞包裹的卵母细胞，在生发泡期被分离出来，并使用添加了0至20微摩尔FF-MAS的培养基进行体外成熟培养。发育至MII期的卵母细胞进行体外受精，并确定发育至2细胞期和囊胚期的百分比。在用于卵母细胞受精或植入前发育的培养基中不添加该固醇。FF-MAS促使所有组中显著更高比例的卵母细胞在体外发育至MII期。此外，对体外成熟的卵母细胞进行FF-MAS处理，除一组外，显著提高了所有卵母细胞完成植入前发育的能力。因此，FF-MAS通过促进体外核成熟和细胞质成熟来提高小鼠卵母细胞质量。
