Kubota Kaname, Ishibashi Takaharu, Matsubara Taku, Hori Tomoyuki, Ozaki Kazuyuki, Yamazoe Masaru, Yoshida Junko, Nishio Matomo, Aizawa Yoshifusa
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, Japan.
J Pharmacol Sci. 2003 Dec;93(4):505-8. doi: 10.1254/jphs.93.505.
We examined the effects of chlorpromazine on NO(3)(-) transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 microg/ml) did not influence NO(3)(-) movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 microM) did not alter NO(3)(-) movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO(3)(-) transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.
我们研究了氯丙嗪对红细胞（RBC）与细胞外液之间硝酸根（NO₃⁻）转运的影响。氯丙嗪（10微克/毫升）对全血和红细胞悬液中的NO₃⁻移动均无影响。尽管阴离子交换蛋白（AE1）抑制剂DIDS（4,4'-二异硫氰酸根合芪-2,2'-二磺酸，100微摩尔）不会改变全血中NO₃⁻的移动，但它在红细胞悬液中以浓度依赖的方式抑制其移动。血浆和白蛋白可浓度依赖性地消除这种抑制作用。我们的结果表明，氯丙嗪对通过AE1的NO₃⁻转运没有影响，并且DIDS在全血中对AE1的无作用是由于血浆中的白蛋白产生了干扰。
Zotero 插件