Penetration of erythrocyte membrane by peroxynitrite: participation of the anion exchange protein.

Participation of the anion exchange protein (Band 3 protein) of the erythrocyte membrane in the transport of peroxynitrite into erythrocytes was shown by partial inhibition of hemoglobin oxidation by extracellular peroxynitrite in cells treated with Band 3 inhibitors. These results demonstrate that permeation in the anionic form may be a minor pathway in the membrane transport of the peroxynitrite anion/peroxynitrous acid couple, especially in membranes reach in anion exchangers or channels.