Kaya Takatoshi, Nagamine Kuniaki, Matsui Nobuto, Yasukawa Tomoyuki, Shiku Hitoshi, Matsue Tomokazu
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
Chem Commun (Camb). 2004 Jan 21(2):248-9. doi: 10.1039/b312462b. Epub 2003 Dec 3.
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.
已经使用电化学传感器芯片实时测量了少数大肠杆菌细胞中的β-半乳糖苷酶表达。使用胶原蛋白凝胶将大肠杆菌细胞包埋在微制造于芯片上的微孔内。以对氨基苯基β-D-吡喃半乳糖苷（PAPG）为底物测定表达的β-半乳糖苷酶的活性。
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