Wiercioch Rafał, Balcerczak Ewa, Byszewska Ewa, Mirowski Marek
Department of Pharmaceutical Biochemistry, Molecular Biology Laboratory, Medical University, Łódź, Poland.
Nucl Med Rev Cent East Eur. 2003;6(2):99-103.
The aim of this study was to examine the biodistribution of (131)I-herceptin in C3H/Bi mice with transplantable mammary adenocarcinoma with a high frequency of C-erbB2 receptor expression.
Mice C3H/Bi with subcutaneously transplanted mammary adenocarcinoma were used as animal model to study the interaction between C-erbB2 receptor and hercepin, a humanized anti-C-erbB2 monoclonal antibody. The expression of the gene encoding C-erbB2 receptor in the tumours was studied by the RT-PCR technique.
Expression of this gene was found in 66% of the studied cases. Similarly, the presence of the C-erbB2 receptor in 77% of the tumours was detected by a Western blot analysis with the use of herceptin. Biodistribution experiments of iodine-labelled herceptin in mice C3H/Bi with adenocarcinoma revealed its maximal accumulation in the tumours at 48 hours since the i.v. injection (7% ID/g). The tumour/muscle radioactivity ratio reached its highest value (above 20) also at 48 hours after the injection.
C3H/Bi mice with this adenocarcinoma may be a good experimental model to study herceptin, or its fragments, labelled with different radionuclids for preliminary evaluation of their usefulness in the therapeutic and diagnostic aspects of breast cancer.
本研究的目的是检测¹³¹I-赫赛汀在C-erbB2受体高表达的可移植性乳腺腺癌C3H/Bi小鼠体内的生物分布。
将皮下移植乳腺腺癌的C3H/Bi小鼠作为动物模型，用于研究C-erbB2受体与赫赛汀（一种人源化抗C-erbB2单克隆抗体）之间的相互作用。采用RT-PCR技术研究肿瘤中编码C-erbB2受体的基因表达。
在所研究的病例中，66%发现该基因表达。同样，使用赫赛汀进行蛋白质印迹分析，在77%的肿瘤中检测到C-erbB2受体的存在。碘标记赫赛汀在患有腺癌的C3H/Bi小鼠体内的生物分布实验显示，静脉注射后48小时，其在肿瘤中的积累量最大（7%ID/g）。注射后48小时，肿瘤/肌肉放射性比值也达到最高值（超过20）。
患有这种腺癌的C3H/Bi小鼠可能是一个很好的实验模型，用于研究用不同放射性核素标记的赫赛汀或其片段，以便初步评估它们在乳腺癌治疗和诊断方面的效用。
