Ishii N, Tsuzuki Y, Matsuzaki K, Miyazaki J, Okada Y, Hokari R, Kawaguchi A, Nagao S, Itoh K, Miura S
Second Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Clin Exp Immunol. 2004 Feb;135(2):226-32. doi: 10.1111/j.1365-2249.2003.02369.x.
Although monocyte-endothelial cell interactions represent an initial step in controlling the recruitment of monocytes in inflamed tissues, their dynamic processes in microvessels of lymphoid (Peyer's patches) and non-lymphoid (villus) regions in gut-associated lymphoid tissue remain poorly understood. We monitored the migration of fluorescence-labelled monocytes derived from the spleen in intestinal microvessels with or without lipopolysaccharide (LPS) treatment and investigated the role of adhesion molecules, P-selectin, vascular cell adhesion molecule (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). In control mice, there were few interactions between infused monocytes and the endothelium of intestinal microvessels. The monocyte-endothelial interactions (both rolling and adhesion) were significantly increased in intestinal microvessels of LPS-treated mice compared with those in controls. Anti-P-selectin monoclonal antibody (MoAb) significantly suppressed the LPS-induced increase in monocyte rolling in postcapillary venules of Peyer's patches and submucosal venules. Anti-VCAM-1 MoAbs significantly suppressed the LPS-induced increase in monocyte adhesion to postcapillary venules (PCVs) of Peyer's patches, submucosal venules, and villus capillaries. In contrast, anti-ICAM-1 MoAb significantly suppressed the number of adherent monocytes in PCV of Peyer's patches but not in submucosal venules or villus capillaries. These observations demonstrated that LPS treatment resulted in a significant increase in recruitment of monocytes both in microvessels of lymphoid and non-lymphoid regions and that P-selectin, VCAM-1 and ICAM-1 appeared to play important roles in LPS-induced interactions.
尽管单核细胞与内皮细胞的相互作用是控制炎症组织中单核细胞募集的第一步，但它们在肠道相关淋巴组织的淋巴样（派尔集合淋巴结）和非淋巴样（绒毛）区域微血管中的动态过程仍知之甚少。我们监测了来自脾脏的荧光标记单核细胞在有或无脂多糖（LPS）处理的肠道微血管中的迁移情况，并研究了黏附分子P-选择素、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的作用。在对照小鼠中，注入的单核细胞与肠道微血管内皮之间几乎没有相互作用。与对照组相比，LPS处理小鼠的肠道微血管中单核细胞与内皮的相互作用（滚动和黏附）显著增加。抗P-选择素单克隆抗体（MoAb）显著抑制了LPS诱导的派尔集合淋巴结毛细血管后微静脉和黏膜下微静脉中单核细胞滚动的增加。抗VCAM-1 MoAb显著抑制了LPS诱导的派尔集合淋巴结毛细血管后微静脉（PCV）、黏膜下微静脉和绒毛毛细血管中单核细胞黏附的增加。相比之下，抗ICAM-1 MoAb显著抑制了派尔集合淋巴结PCV中黏附单核细胞的数量，但对黏膜下微静脉或绒毛毛细血管中的黏附单核细胞数量没有影响。这些观察结果表明，LPS处理导致淋巴样和非淋巴样区域微血管中单核细胞募集显著增加，并且P-选择素、VCAM-1和ICAM-1似乎在LPS诱导的相互作用中发挥重要作用。
