Jiao X, Wang R Y-H, Feng Z, Hu G, Alter H J, W -K Shih J
Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20982, USA.
J Viral Hepat. 2004 Jan;11(1):18-26. doi: 10.1046/j.1352-0504.2003.00464.x.
To induce a sustained and specific cellular immune response to hepatitis C virus (HCV), DNA immunization of mice was performed using plasmids containing the HCV nonstructural gene 3 (HCV/NS3). Plasmids were constructed such that the NS3 gene was expressed in a secreted form, a nonsecreted form or as a membrane-bound antigen. The plasmid encoding the secreted antigen induced the strongest humoral and cellular immunity and favoured the T-helper type 1 (Th1) pathway as shown by cytokine profiles and switching of antibody subclasses. Our study indicates that DNA immunization with a secreted form of HCV/NS3 is an effective means of inducing primary Th1 immune responses in the murine model.
为诱导对丙型肝炎病毒（HCV）产生持续且特异性的细胞免疫反应，使用含有HCV非结构基因3（HCV/NS3）的质粒对小鼠进行了DNA免疫。构建的质粒使NS3基因以分泌形式、非分泌形式或膜结合抗原的形式表达。编码分泌抗原的质粒诱导了最强的体液免疫和细胞免疫，并如细胞因子谱和抗体亚类转换所示，有利于1型辅助性T细胞（Th1）途径。我们的研究表明，用分泌形式的HCV/NS3进行DNA免疫是在小鼠模型中诱导原发性Th1免疫反应的有效手段。
