Ostalska-Nowicka Danuta, Zachwieja Jacek, Nowicki Michał, Witt Martin
Department of Pediatric Cardiology, Poznań University of Medical Sciences, ul. Szpitalna 27/33, 60-572 Poznań, Poland.
Histochem Cell Biol. 2004 Feb;121(2):109-13. doi: 10.1007/s00418-004-0619-7. Epub 2004 Jan 23.
The study attempted to define characteristics of renal podocytes in nephrotic syndrome glomerulopathies in children with and without glomerular immaturity based on the histochemical expression of cytokeratin 18 (CK 18) and vimentin. Material consisted of 29 renal biopsies performed in the Department of Pediatric Nephrology, Poznan University of Medical Sciences, between 1991 and 2000. The study group included 16 children with mesangial glomerulonephritis (MesGN) and signs of glomerular immaturity and 13 children with MesGN without signs of glomerular immaturity. The control tissue was derived from macroscopically normal renal cortex taken from kidneys resected for localised neoplasms ( n=3). In the control samples, the immunocytochemical expression of CK 18 was found only in epithelial cells of proximal and distal tubules. Vimentin was present in all podocytes, some mesangial cells and endothelium. In all cases of children with MesGN with signs of immaturity, both CK 18-positive and vimentin-positive podocytes were found. In all cases of MesGN without immaturity we revealed CK 18-negative podocytes but with distinct vimentin-positive expression. Reorganisation of cytoskeletal proteins within immature podocytes may be associated with the unfavourable clinical course of nephrotic syndrome in children. The application of antibodies against intermediate filaments may help to differentiate between mature and immature forms of MesGN.
该研究试图根据细胞角蛋白18（CK 18）和波形蛋白的组织化学表达，确定患有和未患有肾小球未成熟的儿童肾病综合征肾小球病中肾足细胞的特征。材料包括1991年至2000年间在波兹南医科大学儿科肾病科进行的29例肾活检。研究组包括16例患有系膜增生性肾小球肾炎（MesGN）且有肾小球未成熟迹象的儿童和13例患有MesGN但无肾小球未成熟迹象的儿童。对照组织取自因局部肿瘤而切除的肾脏中肉眼正常的肾皮质（n = 3）。在对照样本中，仅在近端和远端小管的上皮细胞中发现CK 18的免疫细胞化学表达。波形蛋白存在于所有足细胞、一些系膜细胞和内皮细胞中。在所有患有未成熟迹象的MesGN儿童病例中，均发现了CK 18阳性和波形蛋白阳性的足细胞。在所有无未成熟迹象的MesGN病例中，我们发现足细胞CK 18阴性，但波形蛋白有明显的阳性表达。未成熟足细胞内细胞骨架蛋白的重组可能与儿童肾病综合征的不良临床病程有关。应用抗中间丝抗体可能有助于区分MesGN的成熟和未成熟形式。
