Tai Chau Y, Strande Louise F, Eydelman Riva, Sheng Xiaoli, VanTran Jean-Luc, Matthews Martha S, Hewitt Charles W
Department of Surgery, Cooper Health System, UMDNJ-Robert Wood Johnson Medical School, Camden, NJ, USA.
Transplantation. 2004 Jan 27;77(2):316-9. doi: 10.1097/01.TP.0000101511.11171.EF.
An isolated vascularized bone marrow transplant (iVBMT) model was developed to study the contribution of the bone marrow component in a composite tissue allograft. We hypothesized that the iVBMT would be functional and cause graft-versus-host disease (GVHD) in a fraction of the recipients. Lewis iVBMT grafts were transplanted to Lewis-Brown Norway recipients. Animals were sacrificed at various times from 1 to 14 weeks. Polymerase chain reaction for microchimerism was performed on the host's marrow. No animals exhibited signs of GVHD at death. Histologic examination of the grafts showed a normal mix of hematopoietic and fatty elements and appeared to be functional. Tissues usually affected-tongue, ear, liver, and gut-also showed no evidence of disease. Polymerase chain reaction demonstrated microchimerism in both groups. These findings suggest that the vascularized bone marrow within a composite tissue allograft is not the component that causes GVHD; rather, it may serve an immunomodulatory function for tolerance induction.
为了研究复合组织同种异体移植物中骨髓成分的作用，建立了一种孤立的血管化骨髓移植（iVBMT）模型。我们假设iVBMT将发挥功能，并在一部分受体中引发移植物抗宿主病（GVHD）。将Lewis iVBMT移植物移植到Lewis-Brown Norway受体中。在1至14周的不同时间点处死动物。对宿主骨髓进行微嵌合体的聚合酶链反应。没有动物在死亡时表现出GVHD的迹象。移植物的组织学检查显示造血和脂肪成分的正常混合，并且似乎具有功能。通常受影响的组织——舌头、耳朵、肝脏和肠道——也没有疾病迹象。聚合酶链反应在两组中均显示出微嵌合体。这些发现表明，复合组织同种异体移植物中的血管化骨髓不是导致GVHD的成分；相反，它可能具有免疫调节功能以诱导耐受性。
