Labunets I F, Butenko G M, Khavinson V Kh, Magdich L V, Dragunova V A, Pishel' I N, Azarskova M V
Institute of Gerontology AMS Ukraine 67, Vyshgorodskaya st., Kiev-114, 04114 Ukraine.
Adv Gerontol. 2003;12:111-20.
Studies were undertaken on the development of T-lymphocytes in adult and old CBA mice and its changes at aging after injections of pineal gland peptides. It was shown that in old mice the disturbances of T-cells differentiation are registered in bone marrow, thymus, spleen and characterized by the changes of lymphocyte markers expression, migration and proliferation of cells. In old mice FTS titer, melatonin and testosterone levels decreased, the balance of noradrenalin and serotonin in hypothalamus and the cell composition of microenvironment immune systems organs impaired. After chronic (18 mo) administration of the pineal gland preparation epithalamin the amount of stromal cells-precursors, CD4+ and Mac-1(+)-cells in old bone marrow increased, improved the migration of T-cell precursors from bone marrow to thymus and their proliferative potential. The proportion of CD3+, CD4+CD8-, CD4-CD8+, Mac-1(+)-cells in old thymus increased, while that of CD44(+)-cells decreased. The proportion of CD4-CD8(+)-cells in spleen increased. The most number of indices and their balance showed a pattern of adult mice. In old mice after epithalamin the balance of amines in hypothalamus improved, concentration of melatonin in pineal gland, testosterone and FTS titer in blood increased. Epithalon has also the possibility to increase of thymic endocrine function.
对成年和老年CBA小鼠T淋巴细胞的发育及其在注射松果体肽后衰老过程中的变化进行了研究。结果表明，老年小鼠骨髓、胸腺、脾脏中T细胞分化存在紊乱，表现为淋巴细胞标志物表达、细胞迁移和增殖的变化。老年小鼠FTS效价、褪黑素和睾酮水平降低，下丘脑去甲肾上腺素和血清素平衡以及微环境免疫系统器官的细胞组成受损。长期（18个月）给予松果体制剂epithalamin后，老年骨髓中基质细胞前体、CD4+和Mac-1(+)细胞数量增加，改善了T细胞前体从骨髓到胸腺的迁移及其增殖潜力。老年胸腺中CD3+、CD4+CD8-、CD4-CD8+、Mac-1(+)细胞比例增加，而CD44(+)细胞比例降低。脾脏中CD4-CD8(+)细胞比例增加。大多数指标及其平衡呈现成年小鼠的模式。老年小鼠注射epithalamin后，下丘脑胺平衡改善，松果体中褪黑素浓度、血液中睾酮和FTS效价升高。Epithalon还有增加胸腺内分泌功能的可能性。
