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sigma marY1, the LTR of the gypsy-type retroelement marY1 from the basidiomycete tricholoma matsutake, allows multicopy DNA integration in Lentinula edodes.

作者信息

Murata Hitoshi, Miyazaki Yasumasa

机构信息

Department of Applied Microbiology and Mushroom Sciences, Forestry & Forest Products Research Institute, Ibaraki, Japan.

出版信息

Biosci Biotechnol Biochem. 2004 Jan;68(1):218-21. doi: 10.1271/bbb.68.218.

DOI:10.1271/bbb.68.218
PMID:14745187
Abstract

sigma marY1 is the LTR of the retroelement marY1 from the homobasidiomycete Tricholoma matsutake. Upon integration through transformation, pLC1-hph carrying a sigma marY1 derivative, sigma* marY1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* marY1 on Lentinula edodes. Based on the densitometric analysis after Southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter. We conclude that sigma marY1 allows multicopy DNA integration and will be useful in the genetic research on this fungal group.

摘要

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3
Highly polymorphic DNA markers to specify strains of the ectomycorrhizal basidiomycete Tricholoma matsutake based on sigmamarY1, the long terminal repeat of gypsy-type retroelement marY1.
基于gypsy型反转录元件marY1的长末端重复序列sigmamarY1,用于鉴定外生菌根担子菌松茸菌株的高度多态性DNA标记。
Mycorrhiza. 2005 May;15(3):179-86. doi: 10.1007/s00572-004-0319-0. Epub 2004 Sep 16.