Gandía M, Duran-Vila N
Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain.
Arch Virol. 2004 Feb;149(2):407-16. doi: 10.1007/s00705-003-0219-1. Epub 2003 Oct 30.
A field isolate of CBLVd was previously shown to contain two dominant subpopulations (I and II), which differed by the presence or absence of a Sal I restriction site in the PCR product [10]. Here we demonstrate the infectivity and symptom expression of subpopulation II by inoculating Etrog citron with a single representative haplotype. The resulting progeny was characterised as an heterogeneous population of closely related variants with a new fitness peak represented by an haplotype that was not identified in the original isolate. This demonstrates that CBLVd conforms a "quasispecies" model. The progeny shared features of the two subpopulations of the original isolate indicating that the original isolate probably arose from a single CBLVd ancestor.
先前已证明一种CBLVd田间分离株包含两个主要亚群（I和II），这两个亚群在PCR产物中是否存在Sal I限制性酶切位点方面存在差异[10]。在此，我们通过用单个代表性单倍型接种埃塞俄比亚青柠来证明亚群II的感染性和症状表现。所产生的后代被表征为一个由密切相关变体组成的异质群体，具有一个新的适应度峰值，该峰值由原始分离株中未鉴定出的单倍型代表。这表明CBLVd符合“准种”模型。后代具有原始分离株两个亚群的特征，表明原始分离株可能起源于单个CBLVd祖先。
