Wiellette Elizabeth L, Sive Hazel
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Dev Dyn. 2004 Feb;229(2):393-9. doi: 10.1002/dvdy.10464.
Fibroblast growth factor (FGF) signaling is required for normal development of the vertebrate brain, including the isthmus and caudal regions of the hindbrain. Recent work in zebrafish has identified a requirement for the combination of fgf3 and fgf8 functions in specification of rhombomeres 5 and 6 (r5, r6), when evaluated at mid- and late somitogenesis stages. However, when examined earlier in development, during early somitogenesis stages, FGF8 alone is required to initiate r5 and r6 development. Both a mutation in fgf8 and injection of fgf8-targeted antisense morpholino-modified oligonucleotides result in suppression of genes normally expressed in r5 and r6 by the one- to two-somite stage. This expression recovers by the six-somite stage, and we propose that this recovery is a response to activation of fgf3 and to delayed accumulation of fgf8. These data demonstrate an early, nonredundant requirement for fgf8 function in hindbrain patterning.
成纤维细胞生长因子（FGF）信号传导对于脊椎动物大脑的正常发育是必需的，包括后脑的峡部和尾部区域。斑马鱼的最新研究表明，在体节发生的中晚期阶段进行评估时，fgf3和fgf8功能的组合对于菱脑节5和6（r5、r6）的特化是必需的。然而，在发育早期，即体节发生的早期阶段进行检查时，仅FGF8就需要启动r5和r6的发育。fgf8的突变以及注射靶向fgf8的反义吗啉代修饰寡核苷酸都会导致在一到两个体节阶段通常在r5和r6中表达的基因受到抑制。这种表达在六个体节阶段恢复，我们认为这种恢复是对fgf3激活和fgf8延迟积累的反应。这些数据证明了fgf8功能在后脑模式形成中的早期非冗余需求。
