Kirkpatrick J F, Bancroft K, Kincy V
Deaconess Research Institute, Billings, Montana 59102.
J Wildl Dis. 1992 Oct;28(4):590-7. doi: 10.7589/0090-3558-28.4.590.
Sexually mature bison (Bison bison) cows were tested for both pregnancy and ovulation by means of urinary steroid metabolites and fecal steroids. The accuracy of pregnancy diagnosis was determined among 18 bison cows, in approximately the third month of gestation, by means of urinary pregnanediol-3 delta-glucuronide (PdG), urinary estrone conjugates (E1C), and fecal total estrogens (TE). Urinary PdG was 100% accurate, urinary E1C was 89% accurate, and fecal TE was 100% accurate in predicting pregnancy. Fecal progesterone (P4) and TE, as well as urinary E1C and PdG concentrations all increased from conception (August) through January, but significant differences were not apparent until November. During the rutting season ovulation was detected by increases in either urinary PdG or fecal P4 concentrations. Both pregnancy and ovulation were detected in uncaptured bison with reasonable accuracy by means of urinary and fecal steroids or their metabolites.
通过尿液类固醇代谢物和粪便类固醇对性成熟的美洲野牛（Bison bison）母牛进行妊娠和排卵检测。在大约妊娠第三个月时，通过检测18头美洲野牛母牛尿液中的孕二醇-3δ-葡萄糖醛酸苷（PdG）、尿液雌酮结合物（E1C）以及粪便总雌激素（TE），来确定妊娠诊断的准确性。尿液PdG在预测妊娠方面准确率为100%，尿液E1C准确率为89%，粪便TE准确率为100%。从受孕（8月）到1月，粪便孕酮（P4）和TE以及尿液E1C和PdG浓度均有所增加，但直到11月才出现显著差异。在发情季节，通过尿液PdG或粪便P4浓度的增加来检测排卵。通过尿液和粪便类固醇或其代谢物，能够以合理的准确率在未捕获的美洲野牛中检测到妊娠和排卵。
