Orvieto Raoul, Chen Roni, Ashkenazi Jacob, Ben-Haroush Avi, Bar Jacob, Fisch Benjamin
Department of Obstetrics and Gynecology, Rabin Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Petah Tiqva, Israel.
Hum Reprod. 2004 Feb;19(2):357-9. doi: 10.1093/humrep/deh089.
The aim of the present study was to determine serum and follicular fluid C-reactive protein (CRP) levels in patients undergoing controlled ovarian hyperstimulation (COH) for IVF-embryo transfer cycle, and their possible correlation to COH variables.
The subjects were 16 consecutive patients undergoing our routine IVF long GnRH agonist protocol. Blood was drawn three times during the COH cycle: (i) the day on which adequate suppression was obtained (Day-S); (ii) the day of, or prior to HCG administration (Day-HCG); and (iii) the day of (and before) oocyte pick-up (Day-OPU). Levels of sex steroids and serum and follicular fluid CRP were compared among the three time points. Serum and follicular fluid CRP were measured with a commercial immunoturbidimetric assay.
Serum levels of CRP were significantly higher on Day-OPU and Day-HCG than on Day-S, and significantly higher on Day-OPU than on Day-HCG. No difference was observed between follicular and serum CRP levels on Day-OPU. No significant correlations were found between serum and follicular fluid CRP, or between serum CRP-to-BMI ratio and serum sex steroid levels or IVF treatment variables.
The significant increase in serum CRP levels during COH, especially after HCG administration, suggests that COH potentiates a state of systemic inflammation.
本研究旨在测定接受体外受精 - 胚胎移植周期控制性卵巢刺激（COH）患者的血清和卵泡液中C反应蛋白（CRP）水平，以及它们与COH变量之间的可能相关性。
研究对象为连续16例接受我们常规IVF长方案促性腺激素释放激素激动剂的患者。在COH周期中采集三次血样：（i）获得充分抑制的当天（抑制日）；（ii）注射HCG当天或之前（HCG日）；（iii）取卵当天（及之前）（取卵日）。比较三个时间点的性激素水平、血清和卵泡液CRP水平。血清和卵泡液CRP采用商业免疫比浊法测定。
取卵日和HCG日的血清CRP水平显著高于抑制日，且取卵日显著高于HCG日。取卵日卵泡液和血清CRP水平无差异。血清和卵泡液CRP之间，或血清CRP与BMI比值和血清性激素水平或IVF治疗变量之间均未发现显著相关性。
COH期间血清CRP水平显著升高，尤其是在注射HCG后，提示COH增强了全身炎症状态。
