McGeehan J E, Streeter S, Cooper J B, Mohammed F, Fox G C, Kneale G G
Biophysics Laboratories, Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth PO1 2DT, England.
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):323-5. doi: 10.1107/S0907444903026143. Epub 2004 Jan 23.
Single crystals of purified homodimeric controller protein from Aeromonas hydrophilia (C.AhdI) have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from crystals of both the native and a selenomethionine (SeMet) derivative of the protein. The native crystal form belongs to space group P2(1) and data were collected to a resolution of 2.2 A. Two crystal forms of the SeMet protein have been obtained and were found to belong to space groups P1 and P2(1); data have been recorded to 2.0 and 1.7 A resolution, respectively, for the two crystal forms. Three-wavelength MAD data were collected to 1.7 A for the SeMet derivative crystal, which is isomorphous with the native P2(1) crystal.
已使用气相扩散法在几种不同条件下培养出嗜水气单胞菌纯化同二聚体调控蛋白（C.AhdI）的单晶。利用同步辐射从该蛋白的天然晶体和硒代甲硫氨酸（SeMet）衍生物晶体收集了X射线衍射数据。天然晶体形式属于空间群P2(1)，数据收集至2.2 Å分辨率。已获得SeMet蛋白的两种晶体形式，发现它们分别属于空间群P1和P2(1)；两种晶体形式的数据记录分辨率分别为2.0 Å和1.7 Å。对于与天然P2(1)晶体同晶型的SeMet衍生物晶体，收集了至1.7 Å的三波长反常散射数据。
