Erk N
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100, Ankara, Turkey.
Anal Bioanal Chem. 2004 Mar;378(5):1351-6. doi: 10.1007/s00216-003-2427-7. Epub 2004 Jan 28.
The oxidative behaviour of moxifloxacin was studied at a glassy carbon electrode in different buffer systems using cyclic, differential pulse, and Osteryoung square-wave voltammetry. The oxidation process was shown to be irreversible over the entire pH range studied (2.0-10.0) and was diffusion-controlled. The methods were performed in Britton-Robinson buffer and the corresponding calibration graphs were constructed and statistical data were evaluated. When the proposed methods were applied at pH 6.0 linearity was achieved from 4.4 x 10(-7) to 1.0 x 10(-5) mol L(-1). Applicability to tablets and human plasma analysis was illustrated. Furthermore, a high-performance liquid chromatographic method with diode-array detection was developed. A calibration graph was established from 4.0 x 10(-6) to 5.0 x 10(-5) mol L(-1) moxifloxacin. The described methods were successfully employed with high precision and accuracy for estimation of the total drug content of human plasma and for pharmaceutical dosage forms of moxifloxacin.
在玻碳电极上，于不同缓冲体系中，采用循环伏安法、差分脉冲伏安法和奥斯特杨方波伏安法研究了莫西沙星的氧化行为。在所研究的整个pH范围（2.0 - 10.0）内，氧化过程显示为不可逆，且受扩散控制。这些方法在 Britton - Robinson 缓冲液中进行，构建了相应的校准曲线并评估了统计数据。当所提出的方法在pH 6.0下应用时，线性范围为4.4×10⁻⁷至1.0×10⁻⁵ mol L⁻¹。说明了该方法在片剂和人体血浆分析中的适用性。此外，还开发了一种带二极管阵列检测的高效液相色谱法。建立了浓度范围为4.0×10⁻⁶至5.0×10⁻⁵ mol L⁻¹莫西沙星的校准曲线。所描述的方法成功地以高精度和准确性用于估算人体血浆中的总药物含量以及莫西沙星的药物剂型。
