Aminoacylase pellets.
作者信息
Hirano K I, Karube I, Suzuki S
出版信息
Biotechnol Bioeng. 1977 Mar;19(3):311-21. doi: 10.1002/bit.260190303.
PMID:14748
Abstract
Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The aminoacylase pellets were stable in pH 4-8 but they were unstable in alkaline conditions. The aminoacylase pellets were more stable against heavy metal ions and inhibitors than native aminoacylase. However, the degree of the activation of aminoacylase with cobalt ion decreased with the immobilization. It was suggested that most of aminoacylase was covalently coupled to the mycelium with glutaraldehyde.
摘要
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