Park Mi-Kyoung, Kim Duk-Kyu, Lee Hye-Jeong
Departments of Pharmacology, Dong-A University College of Medicine, Busan, Korea.
Exp Mol Med. 2003 Dec 31;35(6):494-500. doi: 10.1038/emm.2003.64.
Hepatocyte growth factor (HGF) is a potent mitogen and promoter of proliferation of insulin producing beta cells of pancreatic islets. To study the role of HGF, an adenoviral vector carrying the human HGF (Ad.hHGF) gene was transfected into the streptozotocin-induced diabetic mice and evaluated the effect on the blood glucose metabolism and the insulin-secreting beta cells of pancreatic islets. Ad.hHGF gene transfection resulted in amelioration of hyperglycemia and prolongation of survival period in the diabetic mice. Concomitantly adenoviral- mediated hHGF gene therapy slightly increased serum insulin concentration and the expression of insulin in the pancreatic islet. Although the proliferation of beta-cell mass was not noticeable, the beneficial effect of HGF is significant to an almost deteriorated pancreatic islets. Taken together, these data suggest that the Ad.hHGF gene therapy into diabetic mice may prevent the further destruction and present as a beneficial remedy for type 1 diabetic patients.
肝细胞生长因子（HGF）是一种强效的有丝分裂原，可促进胰岛中产生胰岛素的β细胞增殖。为了研究HGF的作用，将携带人HGF（Ad.hHGF）基因的腺病毒载体转染到链脲佐菌素诱导的糖尿病小鼠体内，并评估其对血糖代谢和胰岛胰岛素分泌β细胞的影响。Ad.hHGF基因转染可改善糖尿病小鼠的高血糖症状并延长其生存期。同时，腺病毒介导的hHGF基因治疗可使血清胰岛素浓度略有升高，并增加胰岛中胰岛素的表达。尽管β细胞数量的增殖并不明显，但HGF对几乎已恶化的胰岛具有显著的有益作用。综上所述，这些数据表明，对糖尿病小鼠进行Ad.hHGF基因治疗可能会防止其进一步破坏，有望成为1型糖尿病患者的有益治疗方法。
